The Mutation Sites of ASIP Gene and Its Association with Coat Color Phenotype in the Wild Boar Populution
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1.College of Animal Science and Technology, Gansu Agricultural University, Lanzhou,Gansu 730070, China
2.Department of Life Science, Shangqiu Normal College, Shangqiu,Henan 476000, China
The aim of this research was to understand the variation of ASIP function gene influencing animal coat color and association between mutation sites and coat color phenotype in wild boar population. Mutation sites were identified by directly sequencing approach throughout the ASIP gene coding region as well as the 5′ and 3′flanking regions and partial of intronic regions with DNAs from 15 wild boar individuals with different coat color（5 Black, 5 Brown and 5 Silver brown）as template. The results showed that only one mutation site, resulting from single point mutation T to C, was identified in 3′UTR and little mutations in the coding region of ASIP. This SNP was investigated in 72 wild boar belonging to 7 coat color phenotype and 30 Landraces. All mutation was detected in the wild boar population (CC or CT). It was never identified in Landrace population(TT).The results suggest that mutation site C allele identified in the ASIP gene are associated with different coat color phenotype in wild boar population, although the possibility of other coat color function genes determining the coat color phenotype of wild boars can't be ruled out. This results provided theoretical base for further detecting genetic variation of ASIP function gene in wild population.
FU Dong-li,YANG Guang-li, DAI Liao-yuan, LUO Yu-zhu.
The Mutation Sites of ASIP Gene and Its Association with Coat Color Phenotype in the Wild Boar Populution. Journal Of Domestic Animal Ecology. 2013, 34(2): 27-31
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