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  • YOU Junyi, DU Zhaohui, KANG Yahao, DUAN Yu, LIU Xiaowei, YANG Biquan, LU Yongshou, WANG Yuan, LIANG Guodong , MA Yunhui, HU Jianhong, PANG Weijun, SUN Shiduo, LI Xiao
    Journal Of Domestic Animal Ecology. 2024, 45(4): 32-37. https://doi.org/10.3969/j.issn.1673-1182.2024.04.006
    To introduce and select terminal father for pig farms, and select commercial pig combinations that meet the market demand, different lines and breeds, including Danish Duroc, French Duroc, American Duroc and PIC boars were selected and crossed with 2~4 parities of Canadian line binary sows to determine the reproductive performance of sows, daily weight gain of fattening pigs, feed intake, feed-to-meat ratio, death and culling rate, and economic efficiency and other indexes. The results showed in terms of reproductive performance, the total litter size and healthy litter size of Danish Duroc group (DC) were significantly higher than those of American Duroc group (P<0.05). The litter weight of healthy newborns in French Duroc group (FC) was significantly higher than that in American Duroc group (MC) (P<0.05). Average birth weight in MC group was significantly higher than that in PIC group (P<0.05). In terms of growth performance of commercial pigs,DC group had the shortest age of 100kg of correction. The ratio of feed to meat in MC group at 170 days of age was significantly higher than that in PC group (P<0.05). The daily gain at 170 days of age in DC group was significantly higher than that in PC group (P<0.05). The slaughtering weight of DC group was significantly higher than that of the other three groups (P<0.05). The average daily gain in DC group was significantly higher than that in PC group (P<0.05). The ratio of feed to meat in MC group was significantly higher than that in PC group (P<0.05). FC group had the lowest death and culling rate of 2%.Under the same conditions of growth and fattening, Danish Duroc group line has the highest number of litters and has obvious advantage in growth rate and the best economic benefit.
  • JIANG Shaohua, CHANG Xinghai, GAO Yunfan, NAN Feng, MA Dong, LI Shuqin, CHEN Yulin, ZHANG Enping
    Journal Of Domestic Animal Ecology. 2024, 45(3): 55-62. https://doi.org/10.3969/j.issn.1673-1182.2024.03.009
    This study aims to assist in the standardized management of production data in sheep (/goat) farms and the intelligent and scientific application of fine breed breeding. To this end, MySQL database, Java and R languages were used to design a set of sheep(/goat) farm integrated management system software based on Spring Boot+Vue framework. The system optimized the management of sheep(/goat) information, breeding plans, and enabled the visualization of production data. It also included an early warning function to help the sheep (/goat) farm timely grasp the lambing time and production performance of the sheep(/goat). The system is designed with eight modules, including basic sheep(/goat) information management, production information, disease information, breeding information, medication management, feed management, statistical reports and early warning, and scientific breeding. In the scientific breeding management sub-module, three breeding value calculation models (BLUP model, GLMM model, average phenotypic model) were constructed according to the breeding needs of the sheep(/goat) farm. By combining the actual performance of the male and female sheep(/goat), the system achieves the ranking of male and female sheep(/goat) and automatically generates inbreeding coefficients, thus obtaining scientifically reasonable breeding plans. The system was applied in actual production for six months in an intensive mutton sheep(/goat) farm in northern Shaanxi, a joint breeding household for cashmere goat farming in Hengshan District, and a dairy goat farm in Fuping County. By tracking the core population, the system's various functions were improved and optimized. In conclusion, the system provides a data platform for intelligent and digital breeding management in sheep(/goat) farms, and also adds a new version 1.0 to the development of intelligent sheep(/goat) farms.
  • CHE Xiaoyin, YAN Man, SHA Wanli
    Journal Of Domestic Animal Ecology. 2024, 45(3): 88-91. https://doi.org/10.3969/j.issn.1673-1182.2024.03.014
    Pasteurella multocida is a Gram-negative bacterium that infects a wide range of hosts and can cause avian cholera, swine atrophic rhinitis, swine pneumonia, haemorrhagic septicaemia in cattle, sheep and rabbits. With the widespread use of antibiotics, the resistance of pathogenic bacteria is increasing every year. Studies in recent years have even more often found that pathogenic bacteria can develop strong resistance to β-lactams, macrolides, quinolones, tetracyclines, chloramphenicol and sulphonamide antibiotics. This paper summarizes the drug susceptibility and resistance mechanisms of Pasteurella multocida to six types of antibiotics, providing a theoretical basis for the research of new drugs and the establishment of novel therapies.
  • ZHANG Mingfang, WU Guofang, WANG Lei
    Journal Of Domestic Animal Ecology. 2024, 45(8): 1-7. https://doi.org/10.3969/j.issn.1673-1182.2024.08.001
    As a pure natural feed additive, lactic acid bacteria preparations are widely used in animal husbandry because of their functions of promoting animal growth, improving the structure of intestinal flora, improving immune function, and being non-toxic, harmless, and low drug resistance. This review aims to highlight the potential value of lactic acid bacteria in pig, poultry, ruminant and aquaculture to replace antibiotics, in order to provide some reference for the application of lactic acid bacteria in animal production.
  • LIU Ying, ZHANG Jindong, WU Yanli, YANG Yanying, ZHOU Caiquan
    Journal Of Domestic Animal Ecology. 2024, 45(4): 1-7. https://doi.org/10.3969/j.issn.1673-1182.2024.04.001
    With the improvement of human living standards, the demand for meat, eggs and milk has increased significantly, leading to the rapid development of animal husbandry. Antibiotics have been produced largely because they can improve animal functions and prevent diseases. In the context of global antibiotics abuse, livestock and poultry have become important reservoirs for antibiotics and resistance genes. It is urgent to fully understand the distribution and dissemination of antibiotics and resistance genes transmission in the development of animal husbandry in order to provide a reference for the scientific use and rational control of antibiotics in animal husbandry. The article reviews the current status of antibiotic use in animal husbandry. It summarizes the effects of antibiotic resistance gene contamination on livestock, human health, and wildlife. In addition, it emphasizes that future research should focus on the secondary contamination of livestock manure in the surrounding environment and the diffusion mechanism of antibiotic resistance genes in wildlife habitats in animal husbandry. The aim is to provide a reference for the future healthy development of animal husbandry, scientific control of antibiotics, and related research on antibiotic resistance genes.
  • HE Na, SUN Ruizhe, DENG Yuting, LI Mingming, ZHANG Junxia
    Journal Of Domestic Animal Ecology. 2024, 45(6): 1-8. https://doi.org/10.3969/j.issn.1673-1182.2024.06.001
    Mutton is popular among consumers due to its high protein, low cholesterol, and low-fat content. At present, breeders are employing molecular biology to screen potential genes associated with mutton quality in order to provide a foundation for molecular breeding such as mutton quality improvement, hence improving mutton quality. This paper provides a review on studies of candidate genes related to pH, intramuscular fat deposition, and fatty acids in mutton, hoping to render reference for future genetic improvement of sheep, mutton quality improvement, and the development of new breeding techniques.
  • ZHAO Lei, LI Hua, WEI Jindui, CHEN Siyu
    Journal Of Domestic Animal Ecology. 2024, 45(3): 1-7. https://doi.org/10.3969/j.issn.1673-1182.2024.03.001
    Zoonotic diseases have attracted great attentions from relevant social and national departments. Of which, the outbreak of Covid-2019 has further prompted the increasing control over live poultry markets across the country, thereby pushing forward the transition of yellow-feather broilers from live poultry to chilled listing, which has become a major trend. Accordingly, it is increasingly demanding for the carcass integrity rate and aesthetics of yellow-feather broilers after slaughtering, and how to reduce carcass damages of yellow-feather broilers will be a topic afterward. The factors that induce carcass defects after slaughter in yellow-feather broilers are similar to those in white-feathered broilers. However, the alertness and wildness of yellow-feather broilers make them different from white-feather broilers. Fighting, feather pecking or stepping on others can cause carcass defects, which is closely related to social behaviors such as animal fear, anxiety and aggressiveness. Therefore, this paper reviews the research progress on possible factors such as social fear, social competition and stress that induce carcass defects in yellow-feather broilers, and speculates that environmental abundance may be  a feasible improvement measure. It provides theoretical basis for promoting the transition of yellow-feather broilers from live poultry to chilled listing. 
  • WANG Congliang, ZHANG Zhengxuan, BAI Jingjing, FU Qi, LAN Xianyong, CHEN Shenghui, QU Lei, ZHU Haijing
    Journal Of Domestic Animal Ecology. 2024, 45(3): 16-21. https://doi.org/10.3969/j.issn.1673-1182.2024.03.003
    This study aimed to evaluate the correlation between genetic polymorphisms of keratin-associated protein 6.2 gene, growth traits and cashmere fineness of Inner Mongolia cashmere goat, in order to provide a theoretical basis for genetic breeding improvement of Inner Mongolia cashmere goats. Ear tissues of 598 female Inner Mongolia cashmere goats were collected, genomic DNA was extracted and amplified by PCR to detect KAP6.2 gene Insertion/Deletion (InDel) mutation, and the correlation between mutation sites and growth traits and cashmere fineness traits was analyzed. The results showed that in the yearling goat, the mutant was significantly correlated with heart girth and height at hip cross (P<0.05), and extremely significantly correlated with body length, chest width and chest depth (P<0.01); in the adult goat, the mutant was significantly correlated with chest width (P<0.05) and highly correlated with heart girth and height at hip cross and chest depth (P<0.01);in all goat population, the mutant was extremely significantly correlated with body length, heart girth and height at hip cross and chest depth and chest depth (P<0.01), and significantly correlated with cashmere fineness (P<0.05). The above results indicated the 24 bp InDel mutation in the exon region of KAP6.2 gene is significantly and highly significantly correlated with the partial growth traits, and significantly correlated with cashmere fineness in Inner Mongolia cashmere goat, which can be used as a genetic marker for the selection of partial growth traits and cashmere fineness traits. It provides a scientific basis for the selection and breeding of Inner Mongolia cashmere goat.
  • HU Qingquan, SHA Qian, BAO Xiaowei, YANG Rencan, CHANG Yajie, CHEN Jihong, ZHANG Bin, ZHAO Zhiyong
    Journal Of Domestic Animal Ecology. 2024, 45(3): 81-87. https://doi.org/10.3969/j.issn.1673-1182.2024.03.013
    To study the application effect of swine manure treatment with black soldier fly larvae, the study on using black soldier fly larvae to treat swine feces was conducted in four demonstration sites in Yunnan Province, the conversion efficiency and the changes of physical and chemical parameters of pig manure, the nutritional value of black soldier fly larvae and frass were researched. The results showed that the weight loss rate of pig manure was from 59.92% to 71.13%, the total nitrogen reduction rate was 42.83% to 61.73%, and the total phosphorus reduction rate was 42.83% to 61.73%. Black soldier fly larvae was reach in crude protein, ether extract, anmimo acids and fatty acid. The content of crude protein and ether extract were  38.08%~41.04% and 28.27%~33.75% respectively. The content of crude protein and ether extract among the four demonstration sites were significantly different(P<0.05). The content of crude ash in Lufeng and Qujing demonstration sites were of insignificant difference(P>0.05), but it was significantly different between Wuding and Shiping demonstration sites(P<0.05). The difference  of leucine was insignificant (P>0.05), while other amino acids differ significantly in the four sites. The contents of glutamic acid, isoleucine, methionine and histidine were rich. The content of unsaturated fatty acids were 46.65~53.81%, among which the oleic acid and palmitoleic acid were particularly higher, being 28.57% and 16.10%, respectively. The organic matter, total nutrients, the mortality rate of ascaris,pH value,the number of E. coli in frass compost conforms to the national standard of organic fertilizer. The content of organic matter was especially rich, which was 73.87-78.88%, and the total nutrition was 8.20%~9.90%, all of them were higher than the national level. It can be seen that the application effect of swine manure treatment using black soldier fly larvae is remarkable, and the black soldier fly larvae can adapt to the environment well.  It also promotes the innocent treatment and resource utilization of manure and waste from pig farms, realizing the sustainable development of livestock manure and waste. This technology can therefore be popularized in large scale in livestock breeding industry.
  • CHENG Yiyao, YUAN Zhiyu, CHEN Geng, WANG Chunxin
    Journal Of Domestic Animal Ecology. 2024, 45(8): 68-73. https://doi.org/10.3969/j.issn.1673-1182.2024.08.011
    To analyze the advantages and disadvantages of different types of sheep shed environmental monitoring equipment during use, an experimental research on a self-developed multifunctional gas analyzer for livestock and poultry was conducted in terms of the design principles, core technological content, and application. The pros and cons observed during the research and development and application process were then summarized and further optimization concepts and design schemes were proposed. The results show that the first generation of environmental monitoring equipment, due to its fixed nature and limited types of detection data, is more suitable for sheep shed environmental monitoring and early warning; the second-generation equipment, characterized by lightweight and easy operation, is suitable for experiments with small-size data collection and limited types; the third-generation equipment is more suitable for experiments with large-size data collection and multiple types of monitoring.
  • LIANG Wei, SONG Jiashuai, BAO Encai, LIN Yong, GUO Binbin, YING Shijia, WU Zhaoxue
    Journal Of Domestic Animal Ecology. 2024, 45(3): 92-96. https://doi.org/10.3969/j.issn.1673-1182.2024.03.015
    With the development of facility agriculture, the mode of waterfowl breeding is evolving year by year. Because the traditional open aquaculture in water area has become more and more detrimental to poultry' health and ecological environment, waterfowl flat farming in dry land is born in response to it. However, the traditional flat farming in dry land only saves resources and protects the environment. Therefore the battery farming and bio-bedding under slatted floor become an irresistible trend due to their benefits of increasing the land utilization rate and reducing the virus invasion. With the improvement of science and technology, the waterfowl breeding mode has gradually developed from standardization and large-scale to intensification and automation to welfare, and the structure of waterfowl breeding facilities has been gradually improved. The paper investigates the facility structure and breeding mode of waterfowl breeding in various regions of China, summarizes the breeding mode types and history of domestic waterfowl breeding, and predicts its development trend as well.
  • WANG Ruyu, XIAO Haifeng
    Journal Of Domestic Animal Ecology. 2024, 45(3): 46-54. https://doi.org/10.3969/j.issn.1673-1182.2024.03.008
    In order to identify the spatial-temporal evolution characteristics of carbon emissions from mutton sheep breeding in China and explore its emission reduction path, this study adopted partial life cycle method to measure the carbon emissions from mutton sheep breeding in China's provinces (municipalities and autonomous regions) from 2002 to 2019. The LMDI model was established by integrating production efficiency, regional structure, industrial structure, scale effect and technological development into the same research framework. The results show that the carbon emission from gastrointestinal fermentation and fecal management system are the main sources of carbon emissions from mutton sheep breeding, which accounting for 75.58%~90.75%. The carbon emissions of feed grain planting, feed grain processing and mutton sheep raising all show an increasing trend, in particular, the growth rate of feed grain planting is more significant. China's medium and slightly high carbon emission areas of mutton sheep breeding show an obvious dynamic evolution trend, while the spatial pattern of low and high carbon emission areas remain basically unchanged. Scale effect is the largest contributor to carbon emission reduction in mutton sheep breeding, and technology development effect is the main driving factor of carbon emission increase.
  • LIU Menglan, SHAO Xuwen, SHAN Xuesong, JIANG Huaizhi
    Journal Of Domestic Animal Ecology. 2024, 45(7): 1-8. https://doi.org/10.3969/j.issn.1673-1182.2024.07.001
    Ovulation number and litter size are both the key indicators reflecting the reproductive traits of sheep and important economic traits of sheep. As being low to medium heritability traits, they are affected by multiple factors such as genotype, environment and nutritional status. The BMP subfamily, which consists of BMP, GDF and AMH, is an important part of TGF-β superfamily. BMPR1B, BMP15 and GDF9 are currently the most intensively studied genes related to sheep reproductive performance. In this paper, the SNP loci of genes that increase the number of ovulation and litter size of sheep in BMP subfamily members are summarized. The discovery, location, phenotype of SNP loci of these genes affecting reproductive performance, as well as the mechanism of their effects on the number of ovulation and litter size are summed up. The aim is to provide a theoretical reference for the breeding and efficient reproduction of sheep's multiple births.
  • ZHANG Min, LIU Jianxin, WANG Ming, XU Dong, CAI Fula, GAO Yang, ER Xiati, LI Zongxin, HUANG Xixia, QUAN Kaili, AYGL, KUMUSI, ZHENG Wenxin
    Journal Of Domestic Animal Ecology. 2024, 45(3): 22-26. https://doi.org/10.3969/j.issn.1673-1182.2024.03.004
    To investigate the correlation between body weight and body traits in Altay sheep adult ewes, the body weight (y), body height (X1), body length (X2), chest width (X3), chest depth (X4), chest circumference (X5), tube circumference (X6), fat buttock length (X7), fat buttock width (X8), and fat buttock thickness (X9) of 439 healthy and disease-free Altay sheep adult ewes were measured. The data were subjected to descriptive statistics and correlation coefficients using SPSS 26.0 software The path coefficient and stepwise regression in multiple regression analysis were used to establish the optimal regression model for weight and body size. The results showed that there was a highly significant positive correlation (P<0.01) between chest circumference and adult maternal weight of Altay sheep, followed by chest width; the direct and indirect effects of chest circumference on the weight of adult Altay sheep ewes were greater than other traits; The optimal regression model for adult ewes of Altay sheep was Y=0.251X1+0.568X3+0.438X4+3.487X6+0.443X9-42.579. In this model, there was a highly significant linear regression relationship (P<0.01) between body weight and chest circumference, tube circumference, chest width, fat buttocks thickness, and body height. This study elucidated the relationship between the body weight and body traits in Altay sheep adult ewes, which could serve as a reference for actual Altay sheep breeding practices and improve the breeding speed and efficiency of Altay sheep with high body weight.
  • ZHANG Rong, CHEN Guangji , SHANG Yishun , LIANG Baoshan, RAN Weinan, ZHAO Mingkun, BAN Songzhi
    Journal Of Domestic Animal Ecology. 2024, 45(3): 8-15. https://doi.org/10.3969/j.issn.1673-1182.2024.03.002
    The previous researches of the research group found that feeding fresh Fagopyrum dibotrys can maintain the laying rate of laying hens under natural high temperature conditions in summer, but the specific mechanism is unknown. This experiment intended to explore the mechanism of feeding fresh Fagopyrum dibotrys on laying hens from the perspective of intestinal related gene expression and microflora. 300 32-week-old Changshun green shell layers were randomly divided into 5 treatment groups (Group C basic diet, T1 basic diet + 5% fresh Fagopyrum dibotrys, T2 basic diet + 10% Fagopyrum dibotrys, T3 basic diet + 15% fresh Fagopyrum dibotrys, and T4 basic diet + 20% fresh Fagopyrum dibotrys), and each treatment had 5 replicates, 12 layers in each replicate, and the experimental period was 90 days. The results showed: the expression of Occluding and EGF in duodenum and ileum of laying hens in T2 significantly increased(P<0.05). The abundance of bifidobacteria (probiotics) in the ileum and Lactobacillus in the cecum of laying hens of T2 were increased. Therefore, the supplementation of fresh Fagopyrum dibotrys to the diet of laying hens may act on the positive resistance of laying hens and alleviate the stress effect of high temperature environment by up-regulating the expression of Occluding and EGF genes in the intestine and increasing the abundance of beneficial bacteria in the intestine. 
  • GAO Feng, CUI Yaoming, GUAN Junjun
    Journal Of Domestic Animal Ecology. 2024, 45(10): 1-8. https://doi.org/10.3969/j.issn.1673-1182.2024.10.001
    As a major intestinal nutrient, butyric acid and its derivatives play an important role in maintaining animal intestinal morphology, regulating the environment of animal intestinal flora, and regulating immunity. Previous studies have shown that butyric acid and its derivatives maintain the intestinal microenvironment of animals mainly by  promoting intestinal cell proliferation and affecting intestinal development, maintain the intestinal barrier by improving the expression of relevant proteins in small intestinal epithelial cells, and maintain the homeostasis of the internal environment by regulating the intestinal environment and stimulating the expression of relevant immune proteins. In terms of regulating energy metabolism, butyric acid and its derivatives directively break down to become energy supply, or serve as an energy intake regulator to affect energy metabolism by regulating related genes,mitochondrial synthetic repair and neural circuits. In addition, butyric acid and its derivatives act as signaling molecules in the immune pathway, regulate the gene and protein expression of immune factors, inhibit the proliferation of cancer cells, and improve intestinal immunity. This article mainly discusses the research progress of the mechanism of butyric acid in maintaining animal intestinal health, in order to provide theoretical reference for the development of butyrate products.
  • YANG Shaoyan, LIU Hongxiang, CAI Lianshen, ZHANG Yujie, LI Bin, SONG Shanshan, SUN Nana, ZHAO Chenglong, LI Mingyong, LIU Dong
    Journal Of Domestic Animal Ecology. 2024, 45(9): 51-56. https://doi.org/10.3969/j.issn.1673-1182.2024.09.09
    To investigate the cause of caseous exudate in oviducts of growing layers in parts of Shandong,Anhui,Hebei,Ningxia and other regions, bacteria were isolated and cultured from the clinical pathological samples. Morphological,molecular biology,serological,animal regression and drug sensitivity tests for these isolated bacteria were carried out. The results showed that the 13 isolated bacteria were Riemerella anatipestifer, mainly serotype 7, and the OmpA genes had evolved into two branches. The animal regression test could replicate the same clinical symptoms, and the drug sensitivity test showed that the isolated bacteria were more sensitive to florfenicol, doxycycline, spectinomycin and cephalosporins. This study provides a theoretical basis for the isolation and identification of Riemerella anatipestifer from different susceptible hosts and the genetic evolution analysis of strains, and also provides reference for the prevention and control of Riemerella anatipestifer infection in poultry.
  • ZHU Ning, WU Yuhuan, ZHOU Rongzhu
    Journal Of Domestic Animal Ecology. 2024, 45(4): 53-59. https://doi.org/10.3969/j.issn.1673-1182.2024.04.009
    Regional optimization and rational distribution of animal husbandry is an inevitable requirement of agricultural supply-side reform and high-quality development. In this paper, the spatial autocorrelation method is used to analyze the geographical agglomeration characteristics of animal husbandry by using the panel data of provincial animal production in China, and the causes of its formation are analyzed. The results show that the animal husbandry in China presents obvious agglomeration characteristics, and the development of animal husbandry in each province is significantly affected by its neighboring provinces. The spatial agglomeration of industry is unbalanced, with “high-high” agglomeration in eastern and central China and “low-low” agglomeration in western China. Livestock husbandry layout is relatively stable, and it will continue to maintain the status in the future. Based on this, this paper puts forward some policy suggestions, such as promoting moderate scale operation, developing professional, market-oriented and integrated industry chain, and improving supporting system of animal husbandry.
  • ZHANG Xiaoyan, ZHU Li, TANG Lin, ZHAN Yanan, LI Xinpeng, KONG Xiaoyan
    Journal Of Domestic Animal Ecology. 2024, 45(6): 14-19. https://doi.org/10.3969/j.issn.1673-1182.2024.06.003
    Myogenic factor 5 (MYF5) is an important gene affecting muscle growth and development. The purpose of this study was to investigate the effects of genetic variation of MYF5 gene on growth traits of Diannan small-ear pigs, and to provide effective molecular genetic markers for breeding new meat varieties (lines) of high quality Diannan small-ear pigs. Using myogenic factor 5 (MYF5) as candidate gene (Duroc (n=40) as control), the dominant SNP locus of Dian-nan small-ear pigs (n=18) were screened, and the association between the selected dominant SNP and main carcass and meat quality traits  were analyzed.  Results showed among the 16 SNP loci detected in MYF5 gene of Diannan small-ear pigs, 1 is located in the coding region, and 4 dominant SNPs are screened. The results of association analysis showed that the mutation frequencies of 1486, 1574, 1629 and 1701 loci of MYF5 gene are consistent and the genetic map distance is similar, and the linkage is close. The A1486G loci were GG, AG and AA genotypes. The G1574A locus was AA, GA and GG genotypes, respectively. The C1629T loci were TT, CT and CC genotypes. The T1701C locus was CC, TC and TT genotype, respectively. The intramuscular fat content of MM genotype was significantly higher than that of MN genotype (P<0.01), MM genotype was significantly higher than that of NN genotype (P<0.05) and NN genotype was significantly higher than that of MN genotype (P<0.05). In terms of marbling score, MM genotype was significantly higher than NN genotype (P<0.01), MM genotype was significantly higher than MN genotype (P<0.01), MN genotype was significantly higher than NN genotype (P<0.01). It can be inferred that MYF5 gene is the main gene affecting meat quality traits of Diannan small-ear pigs. The results can provide molecular basis for improving meat quality in breeding of Diannan small-ear pigs.
  • MA Meng, WANG Kehua, QU Liang, DOU Taocun, GUO Jun, WANG Xingguo, HU Yuping, LU Jian
    Journal Of Domestic Animal Ecology. 2024, 45(3): 34-38. https://doi.org/10.3969/j.issn.1673-1182.2024.03.006
    In order to explore the growth and development of the length and weight among metatarsal, tibia and femur of white feather broilers with different growth rates, the broilers of slow growth rate (wod168), medium growth rate (wod178) and fast growth rate (wod188 and AA) were taken as the materials to determine the length and weight of their metatarsal, tibia and femur from 2 weeks to 7 weeks. The results showed that the bone traits increased with age from 2 weeks to 7 weeks among all the broilers. The highest growth intensity of metatarsal length, tibial length were observed in wod188 at 3 weeks, with corresponding relative growth coefficients of 36.47% and 25.11%, the greatest growth intensity of femur length, tibial weight and femur weight were in  wod178 at 3-week age, and the corresponding relative growth coefficients were 21.49%,71.32% and 74.49% respectively. The greatest growth intensity of metatarsal weight was in wod168 at 4 weeks of age, and the corresponding relative growth coefficients was 81.66%. At 7 weeks of age, the metatarsal weight of wod168 and wod178 were extremely lower than that of wod188 and AA (P<0.01). The tibial weight of wod178 was remarkably higher than that of wod168, and extremely lower than that of wod188 and AA (P<0.01). The femoral weight of wod168 was significantly lower than the others(P<0.01), there was no significant difference in the other three varieties (P>0.05). The bone growth and development of the four breeds basically reached the maximum at 3 weeks of age, the growth and development rate was the fastest, and broilers with higher blood ties of laying hens have lighter bone weight.
  • WANG Zhen, HAN Yongsheng, ZHANG Shufen, DING Xinying, FU Long, MA Shanshan, YIN Junyi, BAI Changsheng, ZHANG Jun, TIAN Qiufeng, SHI Tongrui, ZHAO Jinbo, LI Wei
    Journal Of Domestic Animal Ecology. 2024, 45(3): 75-80. https://doi.org/10.3969/j.issn.1673-1182.2024.03.012
    The purpose of the study was to alleviate the contradiction between the increase of livestock production capacity and the increasingly serious pollution of livestock manure, to explore the potential of fertilizer utilization of livestock manure and avoid the potential heavy metal hazards to the environment caused by excessive application. The experiment used organic fertilizer made of cow dung as the research object for pot experiment, and five treatments were set up according to different proportions of organic fertilizer made of cow manure and chemical fertilizer application, and the mass fraction of each form of heavy metal Cu and Zn in the soil of different treatments after harvesting was determined, the determination of soil organic carbon and pH value was also performed. The results showed that the distribution of cow dung organic fertilizer could reduce the total mass fraction of heavy metals Cu and Zn in the soil, and the DF1 treatment had the greatest reduction, which was 25.54% and 39.20%, respectively, compared with the CK treatment. Meanwhile, it promoted the transformation of residue state to exchangeable state, the distribution rate of the exchangeable state Cu in DF1 and DF2 treatments increased form 15.86% to 28.13% and 42.02%,respectively, compared with CK treatment, and DF2 treatment had the greatest increase on the distribution rate. Zn distribution rate of residue state was reduced to 39.27% and 38.40%, respectively, in CD and DF1 treatments, compared with CK treatment. The change in DF2 treatment was the greatest, from 53.40% to 24.33%. After the application of cow dung organic fertilizer, the soil pH and organic carbon content had a tendency to increase, in which the soil organic carbon was positively correlated with the trend of the allocation rate of the effective state of Cu and Zn in the soil, and the correlation was significant (P<0.05), while the positive correlation between the soil effective state of Zn and the trend of the soil pH was significant (P<0.05), but the correlation of the effective state of the heavy metal Cu and the pH was not significant (P>0.05). To sum up, the application of organic fertilizer made of cattle manure should strictly follow the actual demand calculated by soil testing formula to avoid excessive application which increases the risk of heavy metal pollution in the soil environment.
  • ZHANG Xiaoran, WANG Pengyu, ZHANG Jun, GENG Xiaoqing, ZHAO Xinlong, ZHANG Yihui, SI Sujin, LI Donghua, TIAN Yadong, JIANG Ruirui
    Journal Of Domestic Animal Ecology. 2024, 45(4): 38-46. https://doi.org/10.3969/j.issn.1673-1182.2024.04.007
    Poultry is one of the animals that needs long time light exposure. Scientific and correct light program has a significant impact on the growth and development, sexual maturity, first birth age, and egg production peak of laying hens. It is of great production significance to set the corresponding light program during the different stages of induced molting in fasting and feeding recovery. In this study, 529-day-old Guifei chickens with similar body weight and about 60% egg production rate were used as experimental material. According to the different periods of fasting induced moult, the changes of light regime were divided into four stages, and samples were collected at five different periods: the last day of the first stage (16 h L/8 h D, Before fasting), stage Ⅱ (8 h L/16 h D for 3 days), and stage Ⅲ (10 h L/14 h D for 13 days) (F0, F3, F16), and 6 d and 32 d of stage Ⅳ (gradually increasing light exposure by 0.5 h/d to 16 h L/8 h D). The number of follicles, ovarian antioxidant indicators, KIT-PI3K-PTEN-AKT signaling pathway and GDF9 gene expression were measured to explore the role of light program in the process of fasting induced molt. Results showed that the levels of FSH, LH, E2 and P4 in serum decreased when light exposure was shortened(P<0.001), and the activities of superoxide dismutase (SOD)(P<0.05) and glutathione peroxidase (GSH-PO) (P<0.001) increased. In particular, primordial follicles were likely to be activated, leading to the increase of numbers of small yellow follicles(P<0.01), primary follicles and secondary follicles(P>0.05). When the light was gradually restored, the expression of KIT and PI3K was continuously high(P<0.001), the expression of PTEN decreased(P<0.01), and the expression of AKT was basically unchanged(P>0.05). The serum levels of reproductive hormones and ovarian antioxidant levels returned to the pre-test state, but the overall number of follicles increased. The results suggest that scientific and reasonable light program during fasting and molting has an auxiliary effect on the remodeling of ovarian function.
  • ZHANG Di, FANG Xiaohuan, LI Junjie, WANG Zhigang
    Journal Of Domestic Animal Ecology. 2024, 45(6): 9-13. https://doi.org/10.3969/j.issn.1673-1182.2024.06.002
    Oocytes vitrification at germinal vesial (GV) stage has unrivaled advantages for freezing oocytes and long-term preservation of local livestock germplasm resources because they do not form the extremely sensitive spindle, which have attracted researchers' wide attention. On the basis of it, this paper reviews the significance of oocyte vitrification cryopreservation of sheep at GV stage. It analyzes the problems in current oocyte cryopreservation process of sheep at GV stage, and summarizes the effective solutions to improve the oocyte freezing efficiency. It provides reference for improving the efficiency of oocyte cryopreservation in the GV stage of sheep and goat.
  • ZHAO Boyu, HAO Peng, ZHANG Jiaqi, XU Xiangzhu, Basang wangdui, GAO Yunhang
    Journal Of Domestic Animal Ecology. 2024, 45(6): 82-87. https://doi.org/10.3969/j.issn.1673-1182.2024.06.013
    The rapid development of livestock and poultry farming is accompanied by the massive use of antibiotics which are excreted through feces and urine because they cannot be completely metabolized. The accumulated antibiotics in feces and urine enter the environment in various ways, thus leading to problems as antibiotic residues and drug resistance. It poses potential threats to human and animal health and the ecological environment, so it is important to effectively remove antibiotics from the environment. However, the removal of antibiotics should not only focus on the degradation effect, but also on secondary pollution, degradation costs and other issues. This paper introduces the sources and transmission of antibiotics and resistance genes in the breeding environment, and highlights the existing methods, principles and characteristics of antibiotic degradation, including physical adsorption, advanced oxidation technology and biodegradation. It also proposes suggestions on the current antibiotic contamination problems in order to provide reference for efficient antibiotic degradation.
  • YANG Xinyao, TANG Kailin, ZHOU Tiantong, CHEN Peiying, LI Manning, CHENG Jiaqi, YIN Yulong, HE Qinghua
    Journal Of Domestic Animal Ecology. 2024, 45(4): 47-52. https://doi.org/10.3969/j.issn.1673-1182.2024.04.008
    Black soldier fly larvae have good application prospects in the resource utilization of kitchen waste. To investigate the effect of spiciness on the growth of black soldier fly larvae, seven different spiciness gradient diets (capsaicin concentration of 0, 0.2, 0.4, 0.6, 0.8, 1.0 and 1.2%) were fed to 7-day-old black soldier fly larvae in this study. The results showed that the feed to gain ratio of the 0.8% capsaicin group was significantly lower than that of the 0.6% capsaicin group (P<0.05), but there was no significant difference with other groups. The larvae in the 0.8% capsaicin group had the lowest dry matter content and were significantly lower than those in the 0.6% and 1.2% capsaicin groups (P<0.05). There were no significant differences in other indicators such as growth performance, survival rate, conversion rate and waste reduction index of black soldier fly larvae (P>0.05). The results showed that the 0.8% capsaicin group had the least nutrients converted from feed, and the 0.6% capsaicin group had the lowest conversion efficiency. Therefore, the larvae of black soldier fly show great tolerance to spiciness, and the results of this study will provide basic data for black soldier fly larvae in the resource utilization of kitchen waste.
  • LI Ning, WU Tiecheng, ZHAO Shuming, WANG Tao, ZHU Lixian, ZHAO Shengguo, LIU Bin
    Journal Of Domestic Animal Ecology. 2024, 45(4): 8-14. https://doi.org/10.3969/j.issn.1673-1182.2024.04.002
    In this study, the genetic parameters of birth weight (BW), birth litter weight (TLW), weaned litter weight (WW), weaned litter weight (TWW), litter size (LZ) and gestation period (GP) of Alxa cashmere goats were estimated using ASreml software combined with constrained maximum likelihood (REML) method. 6517 breeding traits records of Alxa cashmere goat breeding farms from 2017 to 2022 were collected. Eight different single-trait animal models were fitted, and the best model was selected by LRT test. The variance components and heritability of reproductive traits were estimated by the single-trait model, and the phenotypic and genetic correlations between reproductive traits were analyzed by the two-trait model. The results showed that the estimated heritability values of BW, TLW, WW, TWW, LZ and GP of Alashan white cashmere goats were 0.15±0.04, 0.16±0.02, 0.20±0.06, 0.12±0.03, 0.14±0.03 and 0.32±0.05, respectively. The genetic correlation coefficient ranged from -0.49 to 0.73. The highest genetic correlation coefficient between LZ and TLW was 0.73, and the lowest genetic correlation coefficient between LZ and BW was -0.49. The phenotypic correlation ranged from -0.50 to 0.65, among which TWW and TLW had the highest phenotypic correlation coefficient of 0.65, followed by LZ and TLW with the phenotypic correlation coefficient of 0.61, and LZ and BW had the lowest phenotypic correlation coefficient of -0.50. The results showed that the reproductive traits of Alxa cashmere goats were low heritability traits and were not only affected by non-genetic factors such as year and group, but also all the traits were affected by maternal effects. The high correlation between LZ and other reproductive traits indicates that selection based on LZ is effective and can improve the overall reproductive performance of ewes. In the actual production process, the breeding performance of Alxa cashmere goats can be improved by improving the feeding management and nutrition level.
  • HU Zhengyu, ZHANG Haoxin, LIU Yali, GE Wen, WANG Han, JIN Hangfeng, HE Ke, YAN Feifei, ZHAO Ayong
    Journal Of Domestic Animal Ecology. 2024, 45(3): 27-33. https://doi.org/10.3969/j.issn.1673-1182.2024.03.005
    In order to investigate the effect of Dendrobium officinale leaf on laying hens' production performance, egg quality, organ index and serum biochemical index, 240 healthy 19-week-old Jinghong No. 1 laying hens were randomly divided into three treatment groups: control group, 1 200 mg/kg Dendrobium officinale leaf group, and 3 600 mg/kg Dendrobium officinale leaf group. The experimental period was 16 weeks. The results showed that compared with the control group, the addition of Dendrobium leaf dry powder to the diet had no significant effect on the egg production rate, average egg weight and feed-to-egg ratio of Jinghong No. 1 laying hen, indicating that Dendrobium officinale leaves did not affect the egg-laying performance of layer chickens; 3 600 mg/kg Dendrobium officinale leaf could significantly reduce the daily average feed intake aged at 31~34 weeks and egg damage rate of laying hens aged 27 to 34 weeks, and significantly increase the yolk color, serum total protein level and globulin level of laying hens aged 19~22 weeks; 1 200 mg/kg Dendrobium officinale leaves could significantly reduce the daily average feed intake of laying hens aged 31~34 weeks, the egg damage rate of laying hens aged 31~34 weeks, and significantly increase the yolk color and yolk ratio of laying hens aged 27~30 weeks and the Haugh unit of laying hens aged 31~34 weeks. Compared with the other two groups, 3 600 mg/kg Dendrobium officinale leaves significantly increased the spleen index. The results show that the dry powder of Dendrobium officinale leaves does not affect the laying performance of laying hens, however it could improve the egg quality and immune ability. 
  • WANG Shuai, MA Yue, HUANG Jian, MA Pingjun, ZHAO Shanting, ZHU Xiaoyan
    Journal Of Domestic Animal Ecology. 2024, 45(4): 92-96. https://doi.org/10.3969/j.issn.1673-1182.2024.04.015
    With the rapid development of intensified dairy livestock industry, how to avoid the huge impact of heat stress on dairy livestock even on livestock industry has become a challenge requiring urgent attention in intensified farms. Heat stress may lead to a series of changes including decreaced performance, immunity and reproductive performance. It is reported that trace element Chromium (Cr) can inhibit the adverse effects caused by heat stress and mitigate the harm to animals. This paper reviews the regulatory effects and research progress of chromium on heat-stressed dairy animals, based on the research findings of domestic and international scholars.
  • ZHANG Xiaoting, PENG Zhenzhen, GUO Mali, YUAN Chaohai, CAI Yafei, ZHANG Wei
    Journal Of Domestic Animal Ecology. 2024, 45(8): 8-15. https://doi.org/10.3969/j.issn.1673-1182.2024.08.002
    This study aimed to investigate the genetic diversity of local cattle breeds. In this experiment, blood and hair samples were collected in different regions from Wannan cattle, Leiqiong cattle, Naqu yak and Haizi buffalo, and genomic DNA was obtained. mtDNA COI, Cytb and D-loop genes were amplified by conventional PCR technique, and barcode sequences were obtained by Sanger sequencing, and then their genetic information was explored. The results showed that the A/T content of the mitochondrial genes of the four cattle species were significantly higher than the G/C content, with a significant anti-G bias, and all of them showed high genetic diversity, among which the Naqu yak was particularly outstanding. The use of COI and Cytb protein codons also showed a certain preference. The NJ phylogenetic tree constructed based on Cytb gene showed the best performance and was able to cluster the four cattle species better, which was more suitable for the exploration of phylogenetic relationships in this paper.
  • WANG Zhen, HAN Yongsheng, ZHANG Shufen, FU Long, DING Xinying, MA Shanshan, LI Wei, ZHAO Jinbo, DING Deli, LI Qingying
    Journal Of Domestic Animal Ecology. 2024, 45(9): 1-6. https://doi.org/10.3969/j.issn.1673-1182.2024.09.001
    Livestock and poultry manure is a primary target for agricultural non-point source pollution prevention and control. Biochar prepared from livestock and poultry manure possesses advantages such as a porous structure, rich active groups, and high specific surface area. It can serve as an environmentally friendly, low-cost adsorbent material, offering a new direction for the resource utilization of livestock and poultry manure. This paper reviews the preparation process and modification of livestock and poultry manure-based biochar, as well as its current applications in environmental protection fields such as water pollution control and soil improvement. The existing issues in livestock and poultry manure-based biochar research are discussed, and rational suggestions for the prevention and control of environmental pollution using livestock and poultry manure-based biochar are proposed, providing a theoretical basis for the treatment of waste through waste using livestock and poultry manure.
  • YANG Chaoqun, JIA Hongru, TAN Jianbing, YIN Siqi, WU Silin, ZAN Linsen, YANG Wucai
    Journal Of Domestic Animal Ecology. 2024, 45(3): 39-45. https://doi.org/10.3969/j.issn.1673-1182.2024.03.007
    The aim of this study was to investigate the metabolism of melatonin (MT) in beef cattle and the effects of MT on the synchronous estrus rate, conception, blood antioxidant level and reproductive hormones. In this study, firstly, twelve beef cattle were randomly divided into four groups, 0 mg, 0.464 mg, 4.64 mg and 46.4 mg melatonin were injected at 8 o'clock in the morning and the melatonin concentrations at different time pionts were measured. Then, eighty beef cattle were randomly divided into four groups, melatonin of 0 mg, 0.464 mg, 4.64 mg or 46.4 mg was injected during the second injection of cloprostenol sodium and artificial insemination in the treatment, and estrus rate and estrus conception rate were counted. The blood was collected for the determination of plasma antioxidant level and reproductive hormone content. Results showed that after different doses of melatonin injected subcutaneously, plasma melatonin was at a high value and significantly higher than the control group at 0.5 h (P<0.01), then it begen to decline. 0.464 mg melatonin injected subcutaneously into beef cattle was metabolized to the normal level through 8 h, while 4.64 mg or 46.4 mg melatonin to the normal level through 14 h. When melatonin was injected subcutaneously during estrus synchronization, 46.4 mg melatonin injected subcutaneously could significantly improve the estrus rate of beef cattle (P<0.05), and the conception was higher than that of the control group. 4.64 mg or 46.4 mg melatonin could significantly reduce the content of MDA (P<0.05), increase the content of GSH-PX (P<0.05), and significantly increase the content of SOD (P<0.01). 46.4 mg melatonin could significantly increase the concentration of FSH and LH in plasma during mating (P<0.05). 4.64 mg melatonin could significantly increase the concentration of FSH and E2 during mating (P<0.05), and can increase the P4 content on the 7th day after insemination (P<0.05). This study shows that exogenous melatonin can enhance the synchronous estrus rate and conception in two cloprostenol sodium treatments by improving antioxidant level and reproductive hormone concentration of beef cattle, and the effect is best when 46.4mg melatonin is injected subcutaneously into beef cattle.
  • CHONG Yuqing, HE Xiaoming, DENG Wenbao, NONG Shenghu, FANG Yunxia, LIANG Changxin, HE Zuchun, LIU Pingdan, YUE Dan, DENG Weidong, XI Dongmei
    Journal Of Domestic Animal Ecology. 2024, 45(4): 15-21. https://doi.org/10.3969/j.issn.1673-1182.2024.04.003
    To investigate the polymorphisms of the promoter region 23 bp and the first intron region 12 bp insertion/deletion of Wenshan cattle prion protein gene (PRNP), the polymorphism data of PRNP gene 23 bp and 12 bp of Wenshan cattle were genotyped by polymerase chain reaction and agarose gel electrophoresis. Through comparative analysis with the PRNP data of cattle reported in the literature, the resistance of Wenshan cattle to mad cow disease was obtained. Results showed the frequency of the 23 bp allele in the promoter region of the PRNP gene of Wenshan cattle is predominantly represented by the deletion type (0.654). The frequencies of the 12 bp insertion and deletion alleles in the first intron region are 0.913 and 0.087, respectively, and the frequency of the deletion haplotype is very low (0.082). A significant difference (P<0.05) was observed when comparing the frequencies of the 12 bp insertion/deletion alleles in Wenshan cattle with those reported in cases of BSE-affected and healthy cattle from Germany, the United Kingdom, and Switzerland. However, no significant difference (P>0.05) was found in the insertion/deletion allele frequencies between Wenshan cattle and water buffalo. Therefore, it is concluded that the relatively high frequency of the 12 bp insertion allele and the extremely low frequency of the deletion type haplotype (23 bp insertion-12 bp deletion) in Wenshan cattle confer a higher resistance to BSE disease, making them a valuable resource for disease-resistant breeding programs. Analysis of disease resistance in local cattle breeds in China is beneficial for providing data support for molecular breeding of disease resistance in cattle breeds.
  • ZHANG Zhenxing, LI Zhanhong, WANG Bin, SONG Jianling
    Journal Of Domestic Animal Ecology. 2024, 45(3): 68-74. https://doi.org/10.3969/j.issn.1673-1182.2024.03.011
    To analyze the etiology of chicken respiratory disease in Yunnan Province in 2020, 111 mixed samples of lungs, spleen, throat and trachea were collected from 111 chicken farms in Kunming, Honghe, Qujing, Dali, Chuxiong, and Zhaotong. PCR was preformed to detect the nucleic acids of avibacterium paragallinarum, mycoplasma gallisepticum (MG), mycoplasma synoviae (MS), newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian influenza virus (AIV) and omithobacterium rhinotracheale (ORT). Further, some positive PCR products were verified through sequencing. The results showed that positive rates of avibacterium paragallinarum, MG, MS, NDV, IBV, ILTV, ATV, and ORT were 18.92% (21/111), 10.81% (12/111), 5.41% (6/111), 4.50% (5/111), 2.70% (3/111), 39.64% (44/111), 9.00% (10/111), 18.92% (21/111), respectively. Totally, the mixed infections accounted for 32.43% (36/111), and the mixed infection with 2, 3 and 4 pathogens accounted for 18.92% (21/111), 11.71%(13/111) and 1.80% (2/111), respectively.  In general,  the chicken respiratory disease in Yunnan Province in 2020 was caused by many factors, among which avibacterium paragallinarum, ILTV and ORT were the main pathogens.
  • LI Yinshu, ZHOU Yang
    Journal Of Domestic Animal Ecology. 2024, 45(6): 57-64. https://doi.org/10.3969/j.issn.1673-1182.2024.06.009
    With the sudden outbreak of COVID-19 epidemic as the entry point, the outbreak area and the non-outbreak area regarded as a quasi-natural experiment of the impact of some provinces on pork price, the multi-phase DID model was used to investigate the impact of COVID-19 epidemic on pork price in this study. DID-QR model was built to test the processing effect of COVID-19 epidemic on pork price under different initial price growth rates and different institutional environments. The results showed that the outbreak of COVID-19 epidemic led to a sharp rise in pork price, with an average increase of about 10%. The price of pork affected by the epidemic showed a trend of rising, stable, rising again, stable and falling. During the inspection period, the price of pork increased significantly only in the next month, March, June and July, and began to show an obvious downward trend in October. The outbreak of COVID-19 has widened the gap of pork prices in different regions, and the effect of the epidemic on the price is more obvious in the areas where the initial agricultural economic development level is relatively low and the initial economic development level is relatively high. Therefore, according to the heterogeneity of pork prices under different conditions, the government should formulate targeted measures to reduce the impact of sudden external shocks on the pork market by establishing the linkage mechanism of the main links of the industrial chain and improving the public information platform.
  • ZHU Chunhong, LIU Hongxiang, TAO Zhiyun, SONG Weitao, ZHANG Shuangjie,  XU Wenjuan, WANG Zhicheng, LI Huifang
    Journal Of Domestic Animal Ecology. 2024, 45(6): 20-28. https://doi.org/10.3969/j.issn.1673-1182.2024.06.004
    To analyze the composition and diversity characteristics of the gut microbiota of healthy geese and the surface environmental microbiota of goose house, this experiment used healthy adult Taihu geese as the research subjects. Gizzard, proventriculus, duodenum, jejunum, ileum, and cecum contents were collected, and feces from geese and environmental samples were aseptically collected. Bacterial genomes were extracted, and high-throughput sequencing was performed using the IonS5TMXL platform to analyze the abundance and structural characteristics of the gut microbiota of geese and the environmental microbiota of goose houses. The results showed that the species richness of the proventriculus content microbiota was relatively high, significantly higher than the microbiota of the jejunum, ileum, and cecum (P<0.05). The cecum microbiota exhibited the highest diversity, with its Shannon value significantly higher than that of the duodenum, jejunum, and ileum (P<0.05), greater than that of the gizzard and proventriculus microbiota, but the difference was not significant (P>0.05). In terms of α-diversity indicators of the environmental microbiota in goose houses, except for the Simpson value being significantly higher than that of the ileum (P<0.05), the other indicators did not show significant differences compared to the various segments of the digestive tract (P>0.05). β diversity comparisons revealed that the microbiota structures of the duodenum, jejunum, and ileum contents of geese were relatively similar, while the cecum and fecal microbiota had closer genetic and compositional distances. The microbiota structures of the gizzard, proventriculus, and goose house environment were similar. Different absolute dominant microbial species were found in the contents of various segments of the goose digestive tract, with significant differences in abundance. The absolute dominant phyla (relative abundance greater than 5.00%) in the healthy gizzard, proventriculus, and small intestine segments (duodenum, jejunum, and ileum) contents were Firmicutes, Cyanobacteria, and Proteobacteria, with the duodenum also including Campilobacterota. The absolute dominant phyla in the cecum contents were Firmicutes and Bacteroidetes, while in the goose house environment, the absolute dominant phyla were Firmicutes and Proteobacteria. Similarly, there were spatial differences in the distribution of dominant genera, with Bacteroides being the absolute dominant genus in the cecum (25.81%). In conclusion, the spatial distribution of the segments of the goose digestive tract significantly influenced the abundance and diversity of the microbiota, while the diversity of the environmental microbiota in goose houses was relatively consistent with that of the various segments of the digestive tract, with different absolute dominant microbial species colonizing different segments of the digestive tract.
  • TIAN Min, HAN Chengming, LIU Kun, HE Dong, LI Yifan, ZHU Yifan, SHI Huaiping, LI Cong
    Journal Of Domestic Animal Ecology. 2024, 45(10): 36-42. https://doi.org/10.3969/j.issn.1673-1182.2024.10.006
    The aim of this study was to investigate alterations in blood physiological and biochemical parameters of Xinong Saanen dairy goats at different lactation stages and the correlations among these parameters to reflect the physiological and metabolic status and overall health conditions of dairy goats at different lactation stages. Blood samples from 150 Xinong Saanen dairy goats at different lactation stages were collected to determine their blood physiological and biochemical indicators for correlation analysis, in order to explore the influence of non-genetic factors on blood biochemical indicators in Xinong Saanen dairy goats. The findings indicated that: (1) lactation stage significantly influenced various blood parameters such as total protein (TP), albumin (ALB), globulin (GLB), albumin/globulin ratio (A/G), glucose (GLU), total cholesterol (TC), and triglyceride (TG) in Xinong Saanen dairy goats. (2) Significant correlations were observed among TP and GLB, ALB and A/G, ALB and BUN, A/G and GLB, A/G and GLU at different lactation periods (P<0.05). Blood biochemical indicators are significantly affected by lactation periods, and the significant correlations have been observed among the blood biochemical indicators in dairy goats. These findings comprehensively reflect the health and physiological status of dairy goats, providing theoretical references for improving goat milk quality, adjusting nutritional supply, and managing goat farms.
  • ZHAO Li, Solang Quji, Tsering Norbu, Sinian Lausanne, Jigme Gatso, Basan Juza
    Journal Of Domestic Animal Ecology. 2024, 45(8): 22-27. https://doi.org/10.3969/j.issn.1673-1182.2024.08.004
    To explore the effects of diets with different concentrate to forage ratios on the lactation performance, digestion and metabolism, rumen fermentation parameters and immunity of Jersey cattle in Tibet, the experiment adopted a single-factor completely randomized block trial design, with different concentrate to roughage ratios (group Ⅰ, 40∶60;group Ⅱ, 50∶50, and group Ⅲ, 60∶40). Results show:(1) The dry matter intake, milk production and milk protein content of dairy cows in group Ⅲ and Ⅱ were significantly higher than those in group Ⅰ (P<0.05). The ratio of feed to milk in group Ⅱ was significantly lower than that in group Ⅲ and Ⅰ (P<0.05). The milk fat rate of group Ⅲ and group Ⅱ was significantly lower than that of group Ⅰ (P<0.05). (2) The apparent digestibility of DM, NDF, ADF and CP in group Ⅲ and group Ⅱ was significantly higher than that in group Ⅰ (P<0.05). (3) The content of TVFA and the proportion of propionic acid in the rumen fluid of dairy cows in group Ⅲ were significantly higher than those in group Ⅱ (P<0.05), and that of group Ⅱ was significantly higher than group Ⅰ (P<0.05). The contents of NH3-N and MCP in group Ⅲ and Ⅱ were significantly higher than those in group Ⅰ (P<0.05). (4) The TP content of dairy cows in group Ⅲ and Ⅱ was significantly higher than that in group Ⅰ (P<0.05). Group Ⅲ and group Ⅱ had significantly higher content of serum IgA than group Ⅰ (P<0.05). IL-4 content in group Ⅰ was significantly lower than that in other two groups (P<0.05). The level of TNF-α in group Ⅰ was significantly higher than that in group Ⅲ and Ⅱ (P<0.05).In summary, diets with concentrate to roughage ratio of 50∶50 and 60∶40 can significantly increase the milk production of the Jersey cattle, improve the digestibility of nutrients, and improve its immunity and anti-inflammatory ability.
  • GONG Ting, HOU Wenjing, MA Hui, ZHENG Ming
    Journal Of Domestic Animal Ecology. 2024, 45(8): 87-91. https://doi.org/10.3969/j.issn.1673-1182.2024.08.014
    Canine parvovirus infection is one of the important causes of enteritis and death in young dogs, posing significant harm to dogs of all breeds and age groups. It is of great significance to assess the infection status of dogs through specific biomarkers and complementary detection methods, and implement rapid diagnosis and treatment plans for infected dogs. This article summarizes the research progress on biomarkers and detection methods related to canine parvovirus infection both domestically and internationally, aiming to provide references for the detection and treatment of canine parvovirus disease.
  • ZHAO Yilong, HUANG Jinfeng, HE Sanggang, LIU Mingjun
    Journal Of Domestic Animal Ecology. 2024, 45(4): 22-26. https://doi.org/10.3969/j.issn.1673-1182.2024.04.004
    F2 individuals of 462 Texel sheep × Altay sheep were selected in this study, including 229 male lambs and 233 female lambs. All the experimental sheep were reared under uniform conditions until 8 months of age and then slaughtered. After slaughter, the tenderness was measured by C-LM4 tenderness meter. Illumina sheep high density (600K) SNP chip was used for genotyping, and the MLM model of GEMMA software was used for genome-wide association analysis of tenderness traits. After quality control, the remaining 613178 SNPs were used for genome-wide association analysis, and a total of 9 potential SNPs were found to be related to tenderness on chromosomes 2, 6, 23, and 24, respectively. Based on the biological functions of genes and related literature, EREG, AREG and PDE1A genes were speculated to be involved in the biological processes of muscle regeneration and muscle fiber formation, and could be used as important candidate genes affecting tenderness. The aim of this study was to locate SNP sites related to sheep tenderness and their important candidate genes by genome-wide association analysis, so as to provide a scientific basis for improving sheep muscle tenderness by molecular biological methods. 
  • ZHAO Guixin, BAI Heping, TAO Yong, ZHANG Yan, LIU Chang, ZHANG Zhiqiang, WU Tonglei, SHI Qiumei
    Journal Of Domestic Animal Ecology. 2024, 45(3): 63-67. https://doi.org/10.3969/j.issn.1673-1182.2024.03.010
    To determine the cause of the disease in beef cattle on a beef farm in Changli County, Hebei Province, a dominant strain was isolated and purified from the nasal mucus of diseased cattle with obvious respiratory symptoms, and identified by bacterial culture, Gram staining, bacterial biochemical identification, rpoB gene detection and 16S rRNA sequence analysis. The drug susceptibility of the isolate to 18 antibiotics such as tylosin and cefradine was tested by K-B test paper. The test results showed that the isolate was positive for Gram staining of short rod-shaped cocci, and could grow as dry and rough yellowish colonies on blood agar medium, and precipitated in LB liquid medium, with slight turbidity in the upper layer and granular and flaky precipitation in the lower layer. The isolate was identified as having lipase, lysine decarboxylase, β-glucosidase and α-glucosidase activities, and was able to ferment algose, maltose and sucrose, which were consistent with the biochemical characteristics of Corynebacterium xerosis. The homology and consistency of 16S rRNA sequences with Corynebacterium saudium were 99% and 91.6%. It was resistant to seven drugs, such as Tylomycin and cefradine, and sensitive to nine drugs, such as ciprofloxacin and Ennoxacin. The test results show that the isolate is  Corynebacterium xerosis.