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20 March 2025, Volume 46 Issue 3
    

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  • Research progress in the GABA on Ovarian Granulosa Cells
    SHI Shengqi, LIU Ruibing, YUAN Huan, ZHANG Yuli, CHU Guiyan, CAI Chuanjiang
    Journal Of Domestic Animal Ecology. 2025, 46(3): 1-8. https://doi.org/10.3969/j.issn.1673-1182.2025.03.001
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    Gamma-aminobutyric acid (GABA) is an important small molecule non-protein amino acid that serves as a vital neurotransmitter. It plays a significant role in promoting animal growth, regulating appetite and providing stress resistance. Additionally, GABA has been found to be essential in modulating the functions of ovarian granulosa cells (GCs). Granulosa cells are the primary somatic cell population in the ovaries of female animals, and their physiological functions, such as the synthesis of steroid hormones, proliferation, and apoptosis, affect follicular growth and atresia. Research has demonstrated the presence of GABA binding affinity sites on ovarian granulosa cells, indicating that GABA can directly participate in the synthesis of estrogen and progesterone through its receptors. Furthermore, upon binding to its receptors, GABA may also indirectly regulate ovarian granulosa cells via the hypothalamic-pituitary-ovarian (HPO) axis and the adenylate cyclase-cAMP signaling pathway. This review aims to present the research history and current advancements related to GABA and discuss the specific effects of GABA on ovarian granulosa cells and the potential regulatory mechanisms involved, as well as contemplate future research directions. The goal is to provide a theoretical basis for the in-depth study of GABA's role in the regulation of animal reproduction.
  • Polymorphism of PRKACB Gene and Its Correlation with Wool Yield in Angora Rabbits
    REN Yuyao, HAN Xudong, LIU Yan, ZHAO Bohao, CHEN Yang, WU Xinsheng
    Journal Of Domestic Animal Ecology. 2025, 46(3): 9-14. https://doi.org/10.3969/j.issn.1673-1182.2025.03.002
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    The objective of this study was to analyze the genetic polymorphism of PRKACB gene sequence in Angora rabbits and its correlation with production traits (body weight, wool yield), and to identify molecular markers in relation to wool production, and to provide reference for accelerating the breeding of Angora rabbits. Direct sequencing was used to detect SNPs in the PRKACB gene exon and promoter region of 169 Angora rabbits, and SPSS 25.0 software was used to analyze the correlation between SNPs and weight and wool production. Results showed PRKACB gene has 10 exons and 9 introns, with the exon sequences being relatively conserved and no SNPs detected. In the 1366bp promoter sequence, two SNP loci (rs81065607A>C and rs81066149C>T) were found, both containing three genotypes and conforming to Hard-Weinberg equilibrium.The polymorphic information content values of rs81065607A>C and rs81066149C>T were 0.331 and 0.327, indicating moderate polymorphism, suggesting that the PRKACB gene has high genetic variability in the Angora rabbit population. The linkage disequilibrium analysis showed a high linkage between the two SNP loci (r2=0.983). The association analysis showed that the individuals with the CC genotypes of rs81065607A>C had significantly higher wool production than those with AA and AC genotypes（P＜0.05); individuals with the TT genotype of rs81066149C>T had significantly higher wool yield than those with CC and CT genotypes（P＜0.05). It is therefore concluded that the SNP sites rs81065607A>C and rs81066149C>T in the PRKACB gene can be potential molecular markers for wool yield in Angora rabbits, and can be used in molecular breeding of Angora rabbits.
  • Analysis of Microbial Diversity in the Cecum of African Ostrich at Different Age
    JIANG Jinfeng, XIE Yang, ZHNG Yan, ZHOU Feng, LAN Zhongqi, SHEN Shuang, LUO Qingping, WANG Jiaxiang, LI Peng
    Journal Of Domestic Animal Ecology. 2025, 46(3): 15-21. https://doi.org/10.3969/j.issn.1673-1182.2025.03.003
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    The cecum is the main organ where the body breaks down dietary fiber and encourages nutrient fermentation, which has a sizable microbiological population. Several species' cecal microorganisms have been examined and studied, but no such research on the African ostrich has been documented. Hence, in this investigation, the diversity of microorganisms in the caecum of African ostriches at 7, 30, 60, and 180 days of age was examined using metagenomics sequencing technology. The results showed that the flora in the caecum of African ostriches was distributed in 9 phyla, 16 classes, 29 orders, 58 families and 128 genera. With increasing age, the abundance and diversity of microbes in the cecum first increased and then decreased, with the microbial abundance peaking at 60 days of age and microbial diversity being highest at 30 days of age. At the phylum level, Firmicutes and Proteobacteria were the most dominant phyla at 7, 60 and 180 days of age, while the dominant phyla at 30 days of age were Firmicutes, Tenericutes and Bacteroidetes. At the genus level, the dominant bacteria in the cecum of different ages were different. Acinetobacter, Escherichiacoli-Shigella, and Akkermania had the highest abundance at the age of 7 days. Anaeroplasma and Bacteroides were the most abundant at the age of 30 days, Acinetobacter and Pseudomonas were the dominant bacteria at 60 and 180 days old, respectively. There were different bacterial groups in the cecum of each age group. At 7, 30, 60, and 180 days of age, there were 28, 24, 8 and 6 different bacterial groups, respectively, and the number of bacterial groups gradually declined with age. In conclusion, African ostriches have complex and diverse cecal microorganisms, and as they mature, the microbial composition and dominating species will shift. This offers a theoretical foundation for future studies on how aging affects gut microorganisms and how they interact with the body.
  • Effects of Early Supplementation of Different Levels of Composite Probiotic Preparations on Growth Performance, Serum Immune Indicators, and Antioxidant Capacity of Calves
    ZHANG Hongwei, GUO Jianjun, ZHOU Yinghao, ZHANG Lei, ZHANG Tong, TAN Meishu, ZHANG Zhaoxing, PAN Jinlong
    Journal Of Domestic Animal Ecology. 2025, 46(3): 22-27. https://doi.org/10.3969/j.issn.1673-1182.2025.03.004
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    To study the effects of supplementation with different levels of composite probiotic preparations at early stages of growth on the growth performance, blood immune indicators, and antioxidant capacity of calves, 48 healthy lactating calves at the age of 2 weeks were selected  and randomly divided into 3 groups. Each group had 16 replicates, and each replicate had one healthy lactating calf. The groups were control group 1 (basic diet), group 2 (basic diet+0.5% composite probiotic preparation), and group 3 (basic diet+1% composite probiotic preparation), with a pre-feeding period of 7 days and an experimental period of 42 days. The results showed that  the average daily weight gain of lactating calves in Experiment 2 and 3 was significantly higher than that in Group 1 (P<0.05), and their feed to weight ratio was significantly lower than that in Group 1 (P<0.05).And there was no significant difference in the initial weight, final weight, and average feed intake of lactating calves among each group (P>0.05). There was no significant difference in body height and body length between the groups of lactating calves (P>0.05), while the chest circumference in Experiment groups 2 and 3 were significantly higher than those in Group 1 (P<0.05).The levels of IgA, IgG, and IL-2 in the serum of lactating calves in Experiment groups 2 and 3 were significantly higher than those in Group 1 (P<0.05).The content of TNF-α in serum of calves in experiment group 2 was significantly lower than that in experiment group 1 (P<0.05). The activities of SOD and T-AOC in the serum of lactating calves in Experiment groups 2 and 3 were significantly higher than those in Group 1 (P<0.05), while their MDA activity was significantly lower than that in Group 1 (P<0.05). In summary, supplementing different levels of composite probiotic preparations in the early stage can improve the growth performance, immune function, and antioxidant capacity of calves, and adding 0.5% of composite probiotic preparations is more suitable.
  • Effects of Low Protein Small Peptide Nutrition Strategy on Growth Performance, Nutrient Apparent Digestibility and Fecal Microorganisms in Growing Pigs
    WANG Xinyuan, FANG Zihan, ZHANG Boya, YUE Xianghui, WEI Yichen, ZHANG Heliang
    Journal Of Domestic Animal Ecology. 2025, 46(3): 28-34. https://doi.org/10.3969/j.issn.1673-1182.2025.03.005
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    This experiment was conducted to investigate the effects of low protein small peptide nutrition strategy replacing standard diet supplemented with antibiotics on growth performance, nutrient apparent digestibility and fecal microorganisms of growing pigs. Forty-eight healthy 'Yorkshire× Landrace' binary growing pigs with body weight of about 14 kg were selected, half male and half female. According to the litter source and body weight, they were divided into 4 treatments by random block method. Each treatment had 3 replicates, with 4 pigs in each replicate. The control group was fed 18.5% CP standard diet + 100 mg/kg aureomycin, the LP group 16.5% CP low protein diet, the LP+ A group low protein diet + 100 mg/kg aureomycin, and the LP+ SP group low protein diet+ 0.25% small peptide. The results showed that the addition of small peptides to low-protein diets significantly reduced the diarrhea index(P<0.05). Compared with the control group, the dry matter digestibility (P<0.05) and the crude protein digestibility (P<0.01) in LP group significantly reduced, while in LP+ SP group the dry matter digestibility (P<0.01) and the phosphorus digestibility (P<0.05) significantly increased. In comparison to LP group, LP+ A group had significantly increased digestibility of dry matter (P<0.05), LP+ SP group had significantly increased digestibility of dry matter and crude protein (P<0.01), and significantly increased digestibility of phosphorus (P<0.05). Compared with LP+ A group, the dry matter digestibility (P<0.01) and the phosphorus digestibility (P<0.05) in LP+ SP group were both significantly increased. No significant differences were observed in the Alpha diversity index of microorganisms in feces and the number of top four microbial species at the genus level(P>0.05)for the four groups. In summary, the reduction of dietary crude protein level by 2 percentage points had no significant effect on the production performance and fecal microorganisms of growing pigs, but it reduced their nutrient apparent digestibility. The low-protein small peptide nutrition strategy can replace the standard diet supplemented with antibiotics. In low-protein diets, small peptides can replace chlortetracycline.
  • A Comparative Study of Milk Composition and Milk Fatty Acid Composition of Dairy Goats Fed Different Types of Feed
    ZHOU Jingjing, FAN Xiaoxiao, WANG Qirui, YANG Ruohan, DING Xuhong, YANG Li, SUN Xiaoqin
    Journal Of Domestic Animal Ecology. 2025, 46(3): 34-41. https://doi.org/10.3969/j.issn.1673-1182.2025.03.006
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    To understand the difference of milk composition and milk fatty acid composition of dairy goats fed different types of feed, 15 dairy goats in mid-lactation were selected from dairy goats fed concentrated silage feed, concentrated hay feed, concentrated fresh grass feed and fresh grass feed respectively, and milk samples were collected to analyze milk composition and milk fatty acid composition. In the experiment, the concentrate to forage ratio of both concentrated silage feed and concentrated hay feed was about 45∶55 and 50∶50, while the composition of the former was corn silage and alfalfa hay, that of the latter was oat grass and alfalfa hay. Free-range dairy goats were fed grass and supplemented with concentrate, while fully pastured dairy goats grazed in the woods were not fed concentrate supplementation. The results showed  that the milk fat content of fully pastured dairy goats was significantly higher than that of goats fed other types of feed (P<0.05), while the milk protein content of goats fed hay concentrate feed was significantly higher than that of goats fed other types of feed (P<0.05). Compared with dairy goats fed silage concentrate and hay concentrate feed, those fed grass feed had higher contents of C18:3n-3 and CLAc9, t11 in their milk(P<0.05), and lower n-6/n-3 ratios (P<0.05). The milk of fully pastured dairy goats had significantly lower contents of C12:0, C14:0, and C16:0 than those fed silage concentrate and hay concentrate feed (P<0.05). The unsaturated fatty acid (UFA) content in the milk of fully pastured dairy goats was significantly higher than that of goats fed other types of feed (P<0.05), while the atherosclerosis index was significantly lower than that of other feed types (P<0.05). These results show that grass feed helps improve milk fat content, while hay feed helps increase milk protein content. From the perspective of the types and levels of beneficial fatty acids for human health in goat milk, the optimal feed type is full grass feed, followed by grass concentrate feed, then hay concentrate feed, and lastly silage concentrate feed.
  • Effects of Cross Breeding between Dorper Sheep and Tibetan Sheep on Growth, Slaughter Performance and Mutton Quality of F1 Generation
    HU Hai, WANG Guowen, CHENG Xiao, YANG Yanling, XU Shangrong, JIA Gongxue
    Journal Of Domestic Animal Ecology. 2025, 46(3): 42-49. https://doi.org/10.3969/j.issn.1673-1182.2025.03.007
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    The experiment aimed to study the effects of Dorper × Tibetan hybridization on the growth, slaughter performance and meat quality of the offspring. The study conducted hybridization experiments using Dorper sheep and Tibetan sheep as the paternal line and Tibetan sheep as the maternal line. The body weight and body measurement indicators of F1 generation of Dorper-Tibetan sheep ( Duzang F1) were determined and compared to those of Tibetan sheep. In each group, 9 individuals were randomly selected for slaughter experiment, and the indexes of meat quality were assessed. The results showed that the body weight, body length and chest circumference of Dorper × Tibetan F1 were significantly higher than those of Tibetan sheep (P<0.001). Additionally, the pre-slaughter live weight, carcass weight, meat weight, bone weight, slaughter rate, eye muscle area and backfat thickness of Dorper × Tibetan F1 were all significantly greater than those of Tibetan sheep (P<0.001), while the coefficients of the liver, lung and kidney were obviously lower (P<0.05). Except for the shear force of longissimus dorsi (P=0.001) in F1 generation being lower than that of Tibetan sheep (P=0.001), there were no significant differences in the physical properties of the muscle between the two groups. The moisture, crude protein, and ash content of the longissimus dorsi in Dorper × Tibetan F1 decreased significantly, whereas the crude fat content was significantly higher than in Tibetan sheep (P<0.001). Compared to Tibetan sheep, the longissimus dorsi muscle of Duzang F1 showed a decreasing trend in calcium, phosphorus, potassium, and iron content, while zinc and selenium levels increased (P<0.05). The proportions of threonine, valine, methionine, isoleucine, leucine, aspartic acid, and histidine in the longissimus dorsi muscle of Duzang F1 were significantly higher, while the proportions of tryptophan, glutamic acid, serine, and tyrosine were significantly lower (P<0.05). There were no significant differences in the total saturated fatty acid content in the longissimus dorsi muscle; however, the contents of the two main monounsaturated fatty acids, palmitoleic acid and oleic acid, were significantly higher, while the levels of various polyunsaturated fatty acids decreased significantly (P<0.05). These results indicate that Duzang F1 exhibits superior growth and slaughter performance compared to Tibetan sheep, with meat quality indices also being reasonable. The hybrid advantages are significant, making this crossbred combination an ideal choice for the agro-pastoral transitional zone of Qinghai.
  • Effect of Different Feeding Methods on Rumen Microbiota and Enzyme Activity in Nanjiang Yellow Goat
    PAN Cheng, LI Haiyan, ZHANG Xiaoming, ZHANG Zhenzhen, CHEN Yu, ZHU Jiangjiang, ZHAO Wangsheng
    Journal Of Domestic Animal Ecology. 2025, 46(3): 50-58. https://doi.org/10.3969/j.issn.1673-1182.2025.03.008
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    This experiment was conducted to study the effect of differenct feeding methods on rumen emzyme activity,rumen microbiota composition and dirersity of Nanjing Yellow goat. Thirty-two three months old Nanjiang Yellow goats with good body condition and similar body weight were randomly divided into grazing group and housing group, with 16 goats in each group. The grazing group was allowed to graze naturally, while the housing group was fed a complete mixed ration. The trial consisted of a 7-day pre-test period and a 60-day experimental test. At the end of the test, rumen fluid was collected for high-throughput sequencing of rumen microbiota 16S rRNA and enzyme activity determination. The PICRUSt software was used to predict the metabolic functions of rumen microbiota in Nanjiang Yellow goats. The results showed that the activity of rumen α-amylase and pepsin in the housing group was significantly higher than that in the grazing group(P<0.01), while cellulase activity was significantly lower(P<0.01). There was no significant difference in α-diversity of rumen microbiota between the housing and the grazing group, but there was a significant difference in β-diversity(P<0.01). At the phylum level, the relative abundance of Patescibacteria and Desulfobacterota in the rumen microbiota of the grazing group was significantly higher than that in the housing group(P<0.05); at the genus level, the relative abundance of Butyrivibrio in the rumen microbiota of the grazing group was significantly higher than that in the housing group(P<0.01), while the relative abundance of Selenomonas was significantly higher(P<0.05), however, the relative abundance of Ruminococcus and Prevotellaceae_UCG-003 was significantly lower(P<0.05)than that in the housing group. At the KEGG2 level, carbohydrate metabolism was detected to be the most abundant metabolism-related pathway, and at the KEGG3 level, it was found that the metabolism-related enrichment pathways were mainly Selenocompound metabolism, Energy metabolism, and Metabolism of cofactors and vitamins. Spearman correlation, which was used to study the relationship between rumen enzyme activity and rumen microbiota abundance, revealed a significantly positive correlation between α-amylase and the genus Ruminococcus(P<0.05), and a highly significant negative correlation with Prevotellaceae_UCG001(P<0.01). Cellulose activity showed a highly significant positive correlation with the genus Butyrivibrio(P<0.01). In conclusion, different feeding patterns have a certain impact on the rumen microbiota structure and enzyme activity in Nanjiang Yellow goats, and the changes in rumen enzyme activities are correlated to rumen microbiota.
  • Environmental Evaluation of Goat Cave Houses in Northern Shaanxi and Construction of Main Gas Regression Equation
    CHENG Yiyao, WANG Song, YUAN Zhiyu, WU Yujin, CHEN Geng, ZHANG Jinyu, ZHAO Zhuo, ZHAO Yunhui, YANG Yuxin, CHEN Yulin, WANG Chunxin
    Journal Of Domestic Animal Ecology. 2025, 46(3): 59-63. https://doi.org/10.3969/j.issn.1673-1182.2025.03.009
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    This experiment aimed to investigate the variation of environmental parameters in sheep caves and the impact of light control measures on the sheep cave environment, and to optimize the number and placement of points for environmental control. The experiment utilized a multi-functional gas analyzer for livestock and poultry (CN201410008619.7) to monitor harmful gas concentrations, light intensity, wind speed, temperature, and humidity continuously  in the context of reusing abandoned cave dwellings in the northern Shaanxi region. The experimental data collected while the sheep were inside the cave were used for analysis of gas concentration differences, comparison of gas fitting degrees inside the cave, and construction of regression equations for harmful gas concentration variations. Results showed there were significant differences (P<0.05) in methane and carbon dioxide gas concentrations at three points within the cave. Light control had an impact on the variation of harmful gas concentrations in the caves. The trend of changes in harmful gas concentrations varied considerably, with ammonia concentrations lower than those in non-light-controlled caves, and carbon dioxide and methane concentrations higher than those in non-light-controlled cave, but with consistent concentration change trends. Throughout the experiment, the sheep in both caves remained in a comfortable environment without obvious stress reactions, indicating the feasibility of utilizing abandoned cave dwellings for sheep husbandry.
  • Integration Level and Spatiotemporal Evolution Characteristics of the Meat Sheep Industry in Arid and Semi-arid Areas
    LIN Zhenzhen, YANG Zhaoxia, GAO Chenxi, ZHAO Kai
    Journal Of Domestic Animal Ecology. 2025, 46(3): 64-72. https://doi.org/10.3969/j.issn.1673-1182.2025.03.010
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    Improving the integration level of the meat sheep industry in arid and semi-arid areas is an important pathway to achieving the high-quality development of meat sheep industry in China. Based on provincial panel data from 2010 to 2022 in arid and semi-arid regions, this study constructs a theoretical model for the meat sheep industry integration, evaluates the integration level in those regions, and analyzes its temporal and spatial evolution characteristics. The results show that the overall integration level of the meat sheep industry in arid and semi-arid regions, as well as in each province, is relatively low, indicating significant room for improvement. The integration level of the meat sheep industry in various provinces of arid and semi-arid regions exhibits significant regional differences, with distinct advantages and disadvantages. During the study period, the integration level of the meat sheep industry in arid and semi-arid regions and its provinces presented diverse trends, showing increasing, “U”-shaped, and inverted “U” patterns. Finally, based on the actual conditions of each province, countermeasures and suggestions are proposed.
  • Research Progress in Biotoxicity Studies of Microplastics Based on Bibliometric Analysis
    YANG Yufei, LI Pan, XUE Zhen, LU Hongzhao, WANG Ling, WANG Shanshan, ZHANG Tao
    Journal Of Domestic Animal Ecology. 2025, 46(3): 73-82. https://doi.org/10.3969/j.issn.1673-1182.2025.03.011
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    Microplastics have become a major factor in the pollution of oceans, rivers, lakes, and soils, significantly affecting the growth and development of various types of organisms. Ultimately, they pose substantial risks to human health through accumulation and magnification in the food chain. To comprehensively grasp the research hotspots and trends in the toxicology of microplastics, this study employed CiteSpace and VOSviewer software to conduct a visual analysis of relevant literature in the field of microplastic toxicity from the core database of Web of Science and CNKI databases. The results indicate that the field of microplastic toxicity research is currently in a rapid development phase, primarily driven by foreign literature. However, the top three authors and institutions in terms of publication volume are all from China, demonstrating that China has a significant influence in this area. The high-frequency keywords in the field of microplastic toxicity research mainly include biological toxicity, marine environment, ecotoxicity, and toxic effects. Early studies focused on microplastic environmental pollution, which later shifted to ecological toxicological effects and, in recent years, to diseases induced by exposure and their pathogenic mechanisms. It has been established that exposure to microplastics can lead to various diseases in the nervous system, immune system, reproductive system, and metabolic system. Given the current irreplaceability of plastics, this study suggests that future research should focus on the management of microplastic pollution, the development of plastic alternatives, the prevention of microplastic biological toxicity, and the treatment of diseases caused by microplastic damage.
  • Effects of Planting Paspalum wettsteinii under Forest on the Soil Physicochemical Properties and Microbial Diversity
    GAO Chengfang, LI Zhenwu, YING Zhaoyang
    Journal Of Domestic Animal Ecology. 2025, 46(3): 83-90. https://doi.org/10.3969/j.issn.1673-1182.2025.03.012
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    Traditional free-range breeding of Hedian chicken in an under-forest pattern often leads to soil degradation and vegetation destruction. This study investigated the effects of planting Paspalum wettsteinii H. under the forest on soil physicochemical properties, soil microbial bacterial community composition, and soil microbial diversity within the 0~20 cm soil layer. It also analyzed the soil improvement effects of this practice on forest land used for free-range Hedian chicken breeding. The results showed that after one season of planting Paspalum wettsteinii H, soil organic matter, total nitrogen, nitrate nitrogen, ammonium nitrogen, available phosphorus, and available potassium contents increased by 12.4%, 57.8%, 63.9%, 47.1%, 25.8%, and 23.7%, respectively, compared to the control group. Soil lead content decreased by 50.66% compared to the control group. The soil bacterial microbial diversity and richness were significantly higher than those of the control group, with the Chao1 index being 59.2% higher on average and the Shannon index being 10.7% higher on average, both reaching a highly significant level (P<0.01). Planting Paspalum wettsteinii H. under the forest reduced the abundance of Proteobacteria and Chloroflexi phyla while increasing the abundance of Acidobacteria phylum, making it the dominant bacterial phylum, whereas Actinobacteria was the dominant phylum in the control group. Planting Paspalum wettsteinii H. under the forest for one year can significantly enhance soil organic matter, total nitrogen, nitrate nitrogen, ammonium nitrogen, available phosphorus, and available potassium contents. It also reduces soil heavy metal lead content, improves soil microbial diversity, and increases the relative abundance of Acidobacteria phylum, demonstrating a good soil improvement and restoration effect on forest land used for traditional Hedian chicken breeding.
  • Truncated Expression of HN Protein of Newcastle Disease Virus and Establishment of Indirect ELISA Method
    WEI Ling, WANG Siyi, XIONG Rongyuan, LIANG Hong, WANG Huaiyu, LI Caihong, YANG Guolin
    Journal Of Domestic Animal Ecology. 2025, 46(3): 91-95. https://doi.org/10.3969/j.issn.1673-1182.2025.03.013
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    To establish a rapid method for detecting antibodies against Newcastle disease virus (NDV), the HN protein was truncated and expressed by prokaryotic expression technology, and the NDV enzyme linked immunosorbent assay (ELISA) method was established with the expressed recombinant protein. The results showed that the target protein was expressed in the form of inclusion body. Indirect ELISA method was employed to detect the positive sera of six other chicken diseases, and the results were all negative, with the lowest antibody detection titer of 1:10 240. The positive and negative coincidence rate of which with Hemagglutination inhibition test (HI) method were 98.08% and 92.31%, respectively. The positive rate of clinical samples was 91.48%. It indicates that indirect ELISA method can be used for NDV immune antibody detection and vaccine immune efficacy evaluation.
  • Isolation, Identification and Whole Genome Genetic Evolution Analysis of Two Novel Duck Reovirus Strains
    LI Changle, YAO Xinyan, WANG Mingyue, XIAO Yi, ZHANG Xuelian
    Journal Of Domestic Animal Ecology. 2025, 46(3): 96-102. https://doi.org/10.3969/j.issn.1673-1182.2025.03.014
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    To understand the sequence information of novel duck reovirus (NDRV) in Guangdong Province, suspected cases of reovirus infection in ducks from Foshan city and Zhaoqing city in Guangdong province were subjected to detection, isolation, identification and whole genome sequence analysis. Post-mortem examination of the suspected samples revealed irregular hemorrhagic patches in the livers of the infected ducks, as well as severe hemorrhage and necrosis in the spleens and bursa of Fabricius. A laboratory-developed detection method was employed to test the samples, and the NDRV was isolated using LMH cells. Subsequently, the isolated strains were subjected to whole genome sequencing and analysis. The results indicated typical cytopathic lesion on the 3rd generation LMH cells, confirming the presence of NDRV. Full-length amplification revealed that the two isolated NDRV strains had a total length of 23 419 bp, which could be divided into 10 fragments, designated as L1~L3, M1~M3 and S1~S4. Nucleotide and amino acid homology analysis demonstrated the highest homology with domestic NDRV strains, and lower homology with Muscovy Duck reovirus (MDRV) and Avain revirus (ARV). Furthermore, genetic evolutionary relationship showed that the 10 fragments of the two isolated strains were evenly distributed within the major branch of NDRV, confirming that the two isolated strains were both NDRV. This study enriches the sequence information of NDRV and provides a reference for the prevention and control of NDRV in Guangdong, as well as for the research of specific biological products.
  • Effect and Risk Assessment of Animal Manure Pollution in Small and Medium-sized Livestock and Poultry Farms in Xingguo County
    LIN Xiaocui, LIU Ruiping, GUO Xiaobo, HUANG Xing, LIU Aiweng, HU Yan
    Journal Of Domestic Animal Ecology. 2025, 46(3): 103-108. https://doi.org/10.3969/j.issn.1673-1182.2025.03.015
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    This paper involves a field research on small and medium-sized livestock and poultry farms in Xingguo County. Based on statistical data and the emission coefficients of livestock and poultry manure, the quantity of livestock and poultry manure and its pollutant content were estimated. The agricultural load of manure pollutants was assessed using alert values and risk levels, and the potential for biogas production from manure was predicted. The results showed the amount of livestock and poultry raised was 81,900 pig equivalent, with swine accounting for 80.31%, cattle for 13.65%, sheep for 3.54%, rabbits for 0.05%, and poultry for 2.45%. The production of livestock and poultry manure was 111,700 tons in pig equiverlent, with chemical oxygen demand of 3 643 t, biochemical oxygen demand of 3 490 t, ammonia nitrogen of 300 t, total phosphorus of 236 t, and total nitrogen of 653 t. The total estimated biogas production from fermentation was approximately 1.57×106 m3. The overall risk level of farmland pollution caused by livestock and poultry manure was assessed as level I, with no pollution observed. The study indicates that the excretion of livestock and poultry manure has not caused pollution to farmland in the short term, and at the current stage, it is more important to adhere to the principles of reduction, harmlessness, and resource utilization, and advocate the concept of healthy livestock breeding in animal husbandry.
  • Research on the Land Carrying Capacity of Livestock and Poultry Farming Based on the Integration of Planting and Breeding in Laiyang City
    WANG Xinyu, LI Can, CONG Lei
    Journal Of Domestic Animal Ecology. 2025, 46(3): 109-116. https://doi.org/10.3969/j.issn.1673-1182.2025.03.016
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    To evaluate the carrying capacity of livestock and poultry farming land in Laiyang City, and to provide quantitative and reasonable scientific basis for promoting the integration of planting and breeding and promoting the development of green agriculture within the counties, this study collected data and conducted field surveys on villages and livestock leading enterprises in each township of Laiyang City. Based on the balance of nitrogen and phosphorus nutrients in farmland, the study calculated the carrying capacity of livestock and poultry farming land, the current status and warning values of livestock and poultry manure load in Laiyang City and its townships, and analyzed the balance of planting and breeding in each township and the impact of leading livestock enterprises on the main townships. The results showed that the carrying capacity of livestock and poultry farming land in Laiyang City was 2.458 million pig equivalents (in terms of nitrogen) and 1.987 million pig equivalents (in terms of phosphorus), and the carrying capacity of livestock and poultry farming land per unit of agricultural land was 26.5 pig equivalents per hectare (in terms of nitrogen) and 21.4 pig equivalents per hectare (in terms of phosphorus), respectively. The actual livestock and poultry farming quantity in each township of Laiyang City was 983 000 pig equivalents, with a unit farmland livestock and poultry load of 10.6 heads/hm2. The amount of farming in Mudian and Dakuang townships had exceeded the theoretical maximum, and the rest of the 16 townships also had different degrees of potential environmental risks. The total actual livestock and poultry farming quantity in Laiyang City in 2022 amounted to 4.321 million pig equivalents. The unit farmland livestock and poultry load was 46.6 heads/hm2, exceeding the carrying capacity of livestock and poultry farming land. To promote the balance and sustainable development of planting and breeding at the county level, two suggestions are proposed. Firstly, the excess livestock and poultry manure can be transferred to nearby townships when transportation is convenient in order to achieve a balance of planting and breeding within the counties. Secondly, large-scale livestock and poultry farming leading enterprises that cause regional excessive manure pollution must provide organic fertilizer processing services. However, for the sake of efficiency and long-term development, it is necessary to consider moderate-scale farming.
  • Analysis and Thoughts on Accelerating the Development of Whole Industry Chain of Mutton Sheep in Shangluo Shaanxi Province#br#
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    XI Limeng, NIU Yingqi, HONG Liang, YANG Yali, ZHOU Bo
    Journal Of Domestic Animal Ecology. 2025, 46(3): 117-121. https://doi.org/10.3969/j.issn.1673-1182.2025.03.017
    Abstract ( ) Download PDF ( ) Knowledge map Save
    To promote the healthy and sustainable development of mutton sheep industry in Shangluo City, Shaanxi province, an investigation and analysis on current status of the entire industry chain of mutton sheep industry was conducted. The problems in the development of the industry were analyzed as well. Based on the advantages for development in local mutton sheep industry, the paper puts forward the specific measures and suggestions for the integrated development of the whole industry chain of production, processing and marketing, which provides a theoretical basis and decision-making reference for accelerating the high-quality development of mutton sheep industry in Shangluo City.
  • Review of research progress on colony health monitoring based on population and individual analysis
    LEI Chaokai, LU Yuntao, WANG Mengyue, WU Wei, LI Shijuan, LIU Shengping
    Journal Of Domestic Animal Ecology. 2025, 46(3): 122-128. https://doi.org/10.3969/j.issn.1673-1182.2025.03.018
    Abstract ( ) Download PDF ( ) Knowledge map Save
    Honey bees play a significant role in pollination and honey production, making them vital to both humanity and the natural world. In recent years, the health of bee populations has come under severe threat due to Colony Collapse Disorder (CCD), drawing widespread attention from researchers who are attempting to monitor and analyze these populations. This paper reviews monitoring methods and analytical techniques for bees from both population and individual perspectives. Population monitoring of bees includes environmental assessment such as temperature monitoring, humidity monitoring, vibration monitoring, and weight monitoring. Individual monitoring of bees focuses on behavioral assessment using devices such as sensors and cameras. In particular, this paper emphasizes video monitoring methods, analytical techniques, and application scenarios for individual bee monitoring. By categorizing, introducing, and comparing various bee monitoring technologies, this study explores the advantages and disadvantages of different monitoring methods and examines their relationship with bee population health. The findings provide a reference for further research on monitoring the health and behavior of bee populations.
Journal of Domestic Animal Ecology
Monthly ,Founded in 1980
Superintendent: Ministry of Education of the People's Republic of China
Sponsored by: Northwest A&F University
Chief Editor: CHEN Yulin
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ISSN 1673-1182
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