Influence of Different Culture Treatment Methods of Fetal Fibroblasts
on Embryos Developmental Capacity of Porcine Somatic Cells Nuclear Transfer

HUANG Yaqiong1,2, SHI Deshun2, CHEN Xujian1, LI Jiazhou1, ZENG Shiyuan

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Journal Of Domestic Animal Ecology ›› 2013, Vol. 34 ›› Issue (6) : 30-35.

Influence of Different Culture Treatment Methods of Fetal Fibroblasts
on Embryos Developmental Capacity of Porcine Somatic Cells Nuclear Transfer

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Abstract

 Influences of different passage culture' methods, different treatments methods and different cell dissociate culture methods of fetal fibroblasts on embryos developmental capacity of Porcine Somatic Cells Nuclear Transfer were mainly discussed in present research.The results showed:(1)The fusion rate in group of 100% contact and confluence was higher than one of the 70~80% contact group(P<0.05). The cleavage rate of fetal fibroblast in 100% contact and confluence group was higher than one of serum starvation group(P<0.05). Blastocyst rates in groups were of no significant difference(P>0.05). (2)The cleavage rate and the blastocyst rate of reconstructed embryos by using fetal fibroblasts in routine digestion group and 4℃frozen group were markedly higher than the frozen and thawed group(P<0.05). Fusion rates among three groups were not significantly different(P>0.05). The results showed that fetal fibroblasts were not suitable as donor cells after the freezing and thawing treatment.(3)By using culture method of tissue explant and enzymatic digestion, the developmental capacity of reconstructed embryos was discussed. The result also showed that the fusion rate, the cleavage rate and the blastocyst rate of the reconstructed embryos in fetal fibroblasts were not significantly different(P>0.05). In conclusion, 100% contact and confluence is better for culture of fetal fibroblasts in porcine.  It is not optimum for using thawed fetal fibroblasts to do Nuclear Transfer directly in porcine. The culture method of tissue explant and enzymatic digestion can both be used to dissociate and culture fetal fibroblasts in Somatic Cell Nuclear Transfer in porcine.

Key words

somatic cell nuclear transfer / porcine / fetal fibroblasts / embryos / developmental capacity

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HUANG Yaqiong1,2, SHI Deshun2, CHEN Xujian1, LI Jiazhou1, ZENG Shiyuan. Influence of Different Culture Treatment Methods of Fetal Fibroblasts
on Embryos Developmental Capacity of Porcine Somatic Cells Nuclear Transfer. Journal Of Domestic Animal Ecology. 2013, 34(6): 30-35

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