DNA Extraction from Cashmere Goat and Its PCR Amplification

GENG Rong-qing,ZHOU Guang-xian,HAN Xu-feng,LI Wei,LI Bi-bo,WANG Xiao-long,CHEN Yu-lin

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Journal Of Domestic Animal Ecology ›› 2013, Vol. 34 ›› Issue (3) : 13-15.

DNA Extraction from Cashmere Goat and Its PCR Amplification

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Abstract

This study established a rapid DNA extraction method from goat cashmere without hair follicle by using the lysis solution including PCR buffer, MgCl2 and proteinase K. The results showed that it could not only extract mitochondrial DNA and nuclear DNA form cashmere shaft at the same time but also complete PCR amplification efficiently. It would provide a foundation for expanding the application of cashmere samples in individual recognition, genetic resources assessment, molecular evolution, molecular marker assisted breeding and other research areas.

Key words

goat cashmere / mitochondrial DNA / nuclear DNA / DNA extraction / PCR amplification

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GENG Rong-qing,ZHOU Guang-xian,HAN Xu-feng,LI Wei,LI Bi-bo,WANG Xiao-long,CHEN Yu-lin. DNA Extraction from Cashmere Goat and Its PCR Amplification. Journal Of Domestic Animal Ecology. 2013, 34(3): 13-15

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