Comparison on Antioxidant Enzyme Activity of SexingSemen and Routine Semen of Cows

ZHAO Xian-lin,ZAN Lin-sen,TIAN Wan-qiang,HU Jian-hong,WANG Wei-hua

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Journal Of Domestic Animal Ecology ›› 2013, Vol. 34 ›› Issue (1) : 40-43.

Comparison on Antioxidant Enzyme Activity of SexingSemen and Routine Semen of Cows

  • ZHAO Xian-lin,ZAN Lin-sen,TIAN Wan-qiang,HU Jian-hong,WANG Wei-hua
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Abstract

The study was to comprehensively determine the quality of sexing semen through comparing the differences of routine semen and sexing semen on sperm activity, acrosom intact rate and the activity of four antioxidant enzymes in seminal plasma. Dividing with flow cytometry, the sexing semen and routine semen were thawed(37.5 ℃,30 s) to analyze the sperm motility and acrosom intact rate, and the motility of superoxide dismutase(SOD), glutathione peroxidase(GSH), glutathione reductase(GR), catalase(CAT)in seminal plasma were also determined. The results showed that the sperm motility of sexing semen was siginificantly lower than that of the routine semen, and the motility of four antioxidant enzymes in seminal plasma was significantly lower than that of the routine semen, too.

Key words

sexing semen / Holstien bull / frozen semen / sperm motility / antioxidant enzyme activity

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ZHAO Xian-lin,ZAN Lin-sen,TIAN Wan-qiang,HU Jian-hong,WANG Wei-hua. Comparison on Antioxidant Enzyme Activity of SexingSemen and Routine Semen of Cows. Journal Of Domestic Animal Ecology. 2013, 34(1): 40-43

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