In order to screen the polymorphisms of STAT1 promoter in cattle and analyze the effect of SNPs on function elements of promoter,two cattle breeds (Wuchuan Black Cattle and Guizhou Holstein cow) with significant difference in breeds property were selected to construct DNA pools, SNP sites were screened by direct sequencing subsequently.The results showed that 3 single nucleotide polymorphisms (SNPs) were found in the 5' flanking region which included T-537G, T-508A and C+10T. Furthermore, bioinformatics tools were used to predict the core region of the promoter and identify various transcription factors binding sites. It demonstrated that 10 new transcription factors binding sites emerged while 1 previous transcription factors binding sites disappeared based on the SNPs found in this study, the remarkable change of secondary structure of RNA were also detected but the range of CpG islands were not influenced by the analysis of various softwares. This work could lay the experimental foundation for the identification of function of STAT1 promoter.
Key words
STAT1 /
SNP /
promoter /
Guizhou Holstein Cow /
Wuchuan Black Cattle
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
［1］Rawlings J S, Rosler K M, Harrison D A. The JAK/STAT signaling pathway［J］. Journal of Cell Science, 2004, 117(8):1 2811 283.
［2］Deng L, Jiang J, Frank S J. Growth hormoneinduced JAK2 signaling and GH receptor downregulation: role of GH receptor intracellular domain tyrosine residues［J］. Endocrinology, 2012,153(5):2 3112 322.
［3］Calo V, Migliavacca M, Bazan V, et al. STAT proteins: from normal control of cellular events to tumorigenesis［J］. J Cell Physiol, 2003, 197(2):157168.
［4］Lanning N J, CarterSu C. Recent advances in growth hormone signaling［J］. Reviews in endocrine & metabolic disorders, 2006, 7(4):225235.
［5］Herrington J, Smit L S, Schwartz J, et al. The role of STAT proteins in growth hormone signaling［J］. Oncogene, 2000,19(21):2 5852 597.
［6］Cobanoglu O, Zaitoun I, Chang Y, et al. Effects of the signal transducer and activator of transcription 1 (STAT1) gene on milk production traits in Holstein dairy cattle［J］. Journal of Dairy Science,2006,89(11):4 4334 437.
［7］Tamura G, Yin J, Wang S, et al. ECadherin gene promoter hypermethylation in primary human gastric carcinomas［J］. Journal of the National Cancer Institute, 2000, 92(7):569573.
［8］Darnell Jr J E. STATs and gene regulation［J］. Science,1997,277(5332):1 6301 635.
［9］Kiu H, Nicholson S. Biology and significance of the JAK/STAT signalling pathways［J］. Growth factors,2012,30(2):88106.
［10］Khatib H, Huang W, Mikheil D, et al. Effects of signal transducer and activator of transcription (STAT) genes STAT1 and STAT3 genotypic combinations on fertilization and embryonic survival rates in Holstein cattle［J］. Journal of Dairy Science,2009,92(12):6 1866 191.
［11］ 褚 敏, 昝林森. STAT1 基因单核苷酸多态性与中国荷斯坦奶牛产奶性状的相关性研究［J］. 畜牧兽医学报,2009,40(3):309314.
{{custom_fnGroup.title_en}}
Footnotes
{{custom_fn.content}}
PDF(916 KB)
