In this case, α1Hb cDNA sequence was isolated and identified from skin cDNA library of Chinese giant salamander. The α1Hb gene was 594 bp in the full length with the ORF 432 bp. Besides, 144 kinds of amino acids were encoded byα1Hb gene, and the molecular weight was 16 048.3 u in general, isoelectric point was 6.44. In addition, the structure analysis of α1Hb showed that the composition of amino acids were acidic amino acids 11.9%, neutral amino acids 69.9% and basic amino acid 18.2%. Structural analysis indicated that there was no signal peptide was existed in this protein but there was an outward transmembrane helical region from 100 th to 122 nd aa and also a inward transmembrane helical region from 98 th to 122 nd aa, whose secondary structure was mainly αhelix.The threelevel structure ofα1HB protein indicated the evolutional relationship was that Andrias davidianus was homologous to land animals, with Homo sapiens and Macaca mulatta homology of α1HB was nearly 50%, and Rana catisbianna took the lowest homology (35%). Moreover, we were able to analyse the expression of α1Hb gene mRNA in 7 tissues for Andrias davidianus by the data of qRTPCR, it was more expressed in muscle than the other six issues.
Key words
andrias davidianus /
alpha-1 hemoglobin /
bioinformatics analysis /
tissue expression
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
［1］朱 玲. 贵州省大鲵资源现状及其保护对策［J］. 中国水产，2006(4): 64-65.
［2］牟洪民，李 媛，姚俊杰，等. 大鲵生物学研究的新进展［J］. 水产科学，2011, 30(8): 513-516.
［3］赖 仞，赵 宇，刘 衡，等. 两栖类动物皮肤活性物质的利用兼论中国两栖类资源开发的策略［J］， 动物学研究，2002, 23(1): 65-70.
［4］Ishimori K, Imai K, Miyazaki G, et al. Sitedirected mutagenesis in hemoglobin: functional and structural role of interand intrasubunit hydrogen bonds as studied with 37 beta and 145 beta mutations［J］. Biochemistry，1992, 31(12): 3 256-3 264.
［5］Hodson D, Hirsch J G. The antibacterial activity of hemoglobin［J］. J Exp Med，1958, 107(2): 167-183.
［6］Takagi H, Shiomi H, Ueda H, et al. A novel analgesic dipeptide from bovine brain is a possible Metenkephalin releaser［J］. Nature， 1979, 282(5737): 410-412.
［7］Fogaca A C, Da S P J, Miranda M T, et al. Antimicrobial activity of a bovine hemoglobin fragment in the tick Boophilus microplus［J］. J Biol Chem，1999, 274(36): 25 330-25 334.
［8］Liu J M, Lawrence F, Kovacevic M, et al. The tetrapeptide AcSDKP, an inhibitor of primitive hematopoietic cell proliferation, induces angiogenesis in vitro and in vivo［J］. Blood，2003, 101(8): 3 014-3 020.
［9］Myers M D, Sosinowski T, Dragone L L, et al. Srclike adaptor protein regulates TCR expression on thymocytes by linking the ubiquitin ligase cCbl to the TCR complex［J］. Nat Immunol，2006, 7(1): 57-66.
［10］Kalka C,Tehrani H,Laudenberg B.VEGF gene transfer mobilizes endothelial progenitor cells in patients with inoperative coronary disease［J］.Ann Thorac Surg,2000,70:829-834.
［11］Ton C,Stamatiou D,Liew CmC. Gene expression profile of zebrafish exposed to hypoxia during development［J］.Physiol Genomics, 2003,13:97-106.
［12］Mendez E, FernandezLuna J L, Grubb A, et al. Human protein HC and its IgA complex are inhibitors of neutrophil chemotaxis［J］. Proc Natl Acad Sci U S A,1986, 83(5): 1 472-1 475.
［13］Roesner A, Mitz S A, Hankeln T, et al. Globins and hypoxia adaptation in the goldfish, Carassius auratus［J］. FEBS J,2008, 275(14): 3 633-3 643.
［14］Wawrowski A, Gerlach F, Hankeln T, et al. Changes of globin expression in the Japanese medaka (Oryzias latipes) in response to acute and chronic hypoxia［J］. J Comp Physiol B,2011, 181(2): 199-208.
［15］Chan F Y, Robinson J, Brownlie A, et al. Characterization of adult alphaand betaglobin genes in the zebrafish［J］. Blood,1997, 89(2): 688-700.
{{custom_fnGroup.title_en}}
Footnotes
{{custom_fn.content}}
PDF(1055 KB)
