Isolation and Purification of Male Germline Stem Cells in Pigs

HE Ying,AN Jun-hui,ZHENG Yi,ZENG Wen-xian

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Journal Of Domestic Animal Ecology ›› 2012, Vol. 33 ›› Issue (4) : 1-4.

Isolation and Purification of Male Germline Stem Cells in Pigs

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Abstract

In order to develop techniques for isolation and purification of male germline stem cells (MGSCs), differential plating and seminiferous tubuleculture were applied to enrich MGSCs from newly born porcine testis tissue. The results showed that MGSCs with high vitality could be obtained via the two methods. MGSCs population enriched to 36.9% and 40.7% respectively, via differential plating or seminiferous tubuleculture. Both techniques can be used for isolation and purification of porcine MGSCs. 

Key words

pig / male germline stem cells / purification

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HE Ying,AN Jun-hui,ZHENG Yi,ZENG Wen-xian. Isolation and Purification of Male Germline Stem Cells in Pigs. Journal Of Domestic Animal Ecology. 2012, 33(4): 1-4

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