The Efficiency of YCFA Medium to Bacteria Enrichment Culturing in Goat's Rumen Based on 16S rRNA Sequencing

GUO Mengmeng, JING Ruoxi

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Journal Of Domestic Animal Ecology ›› 2025, Vol. 46 ›› Issue (4) : 51-57. DOI: 10.3969/j.issn.1673-1182.2025.04.008

The Efficiency of YCFA Medium to Bacteria Enrichment Culturing in Goat's Rumen Based on 16S rRNA Sequencing

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Abstract

This study aimed to screen the cultivable microbial strains from the goat rumen through in vitro enrichment using YCFA medium, providing a potential medium for the in vitro cultivation of rumen microorganisms. Microorganisms from the goat rumen were enriched under anaerobic and aerobic conditions at 37 ℃ using YCFA medium. DNA was extracted from the enriched colonies and subjected to 16S rRNA gene sequencing to analyze the types of bacteria that could be enriched and cultivated. The results showed that YCFA medium under anaerobic conditions could potentially enrich rumen microorganisms such as Veillonella, Streptococcus, Anaerovibrio, Selenomonas_1, Prevotella_1, Prevotellaceae_YAB2003_group, Schwartzia, and Phocaeicola. Under aerobic conditions, the medium could potentially enrich microorganisms such as Mannheimia, Bacteroides, Bacillus, and Actinobacillus. This experiment predicts that certain effective microbial strains from the goat rumen can be cultivated in YCFA medium. These findings serve as a reference for the cultivation of bacterial strains from goat rumen.

Key words

goat rumen microorganisms / 16S rDNA sequencing / YCFA medium / culture through enrichment

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GUO Mengmeng, JING Ruoxi. The Efficiency of YCFA Medium to Bacteria Enrichment Culturing in Goat's Rumen Based on 16S rRNA Sequencing. Journal Of Domestic Animal Ecology. 2025, 46(4): 51-57 https://doi.org/10.3969/j.issn.1673-1182.2025.04.008
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