通过比较常规精液和性控精液在活力、顶体完整率及精浆中四种抗氧化酶类活力的差异，对性控精液的品质进行综合评定。对用流式细胞仪分离后的性控精液和常规精液细管解冻（37.5℃，30s），分别分析精子活力和精子顶体完整率，并测定精浆中的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH）、谷胱甘肽还原酶（GR）和过氧化氢酶（CAT）的活性。结果表明性控精液精子在活力显著低于常规精液，且精浆中四种抗氧化酶类的活力也都显著低于常规精液。
Abstract
The study was to comprehensively determine the quality of sexing semen through comparing the differences of routine semen and sexing semen on sperm activity, acrosom intact rate and the activity of four antioxidant enzymes in seminal plasma. Dividing with flow cytometry, the sexing semen and routine semen were thawed(37.5 ℃,30 s) to analyze the sperm motility and acrosom intact rate, and the motility of superoxide dismutase（SOD）, glutathione peroxidase（GSH）, glutathione reductase（GR）, catalase（CAT）in seminal plasma were also determined. The results showed that the sperm motility of sexing semen was siginificantly lower than that of the routine semen, and the motility of four antioxidant enzymes in seminal plasma was significantly lower than that of the routine semen, too.
关键词
性控精液 /
荷斯坦公牛 /
冷冻精液 /
精子活力 /
抗氧化物酶活性
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Key words
sexing semen /
Holstien bull /
frozen semen /
sperm motility /
antioxidant enzyme activity
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参考文献
［1］Garner D L, Seidel Jr G E. History of commercializing sexed semen for cattle［J］. Theriogenology,2008,69:88695.
［2］Bodmer M, Janett F, Hassig M,et al. Fertility in heifers and cows after low dose insemination with sexsorted and nonsorted sperm under field conditions［J］. Theriogenology,2005,64:1 6471 655.
［3］Schenk J L, Seidel Jr G E. Pregnancy rates in cattle with cryopreserved sexed spermatozoa: effects of laser intensity, staining conditions and catalase［J］. Soc Reprod Fertil,2007,64:165177.
［4］Aitken R J, West, K M. Analysis of the relationship between reactive oxygen species production and leukocyte infiltration in fractions of human semen separated on percoll gradients［J］. Int J Androl,1990,3:433451.
［5］Hu J H, Zhao X L, Tian W Q,et al.Effects of vitamin E supplementation in the extender on frozenthawed bovine semen preservation［J］. Animal，2011, 5(1)：107112.
［6］Flaherty C, Beconi M, Beorlegui N. Effect of natural antioxidants, superoxide dismutase and hydrogen peroxide on capacitation of frozenthawed bull spermatozoa［J］. Andrologia, 1997,29:269275.
［7］Hu J H, Zan L S，Zhao X L,et al. Effects of trehalose supplementation on semen quality and oxidative stress variables in frozenthawed bovine semen［J］. J Anim Sci,2010,88:1 6571 662.
［8］张忠诚.家畜繁殖学［M］.北京：中国农业出版社，2004.
［9］Mariola Stowinska， Halina Karol， Andrzej Ciereszko.Comet assay of fresh and cryopreserved bull spermatozoa.Cryobiology,2008,56:100102.
［10］Hu J H, Tian W Q, Zhao X L, et al. The cryoprotective effects of ascorbic acid supplementation on bovine semen quality［J］.Anim Reprod Sci,2010,121:7277.
［11］Jones R, Mann T. Damage to ram spermatozoa by peroxidation of endogenous phospholipids［J］. Reprod Fertil,1977,50:261268.
［12］赵红光，刘光伟，刘淑春，等.患糖尿病12周大鼠睾丸生精细胞周期及凋亡与抗氧化水平的变化［J］.中华男科学杂志，2005，11（10）：735739.
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脚注
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基金
陕西省科技攻关项目（2004K01-G1）；农业部科技跨越计划项目（跨2007-23）；“十一五”国家科技支撑计划奶业重大专项（2006BAD04A11）;“十二五”国家科技支撑计划（2012BAD12B07）
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