STAT1基因启动子区多态性研究

焦仁刚,杨永强,龚俞,惠嫣婷,刘若余*

PDF(916 KB)
欢迎访问《家畜生态学报》官方网站!
PDF(916 KB)
家畜生态学报 ›› 2013, Vol. 34 ›› Issue (1) : 15-20.
科学研究

STAT1基因启动子区多态性研究

  • 焦仁刚,杨永强,龚俞,惠嫣婷,刘若余*
作者信息 +

Study on the Polymorphism of Promoter Region of STAT1 Gene in Cattle

  • JIAO Ren-gang,YANG Yong-qiang,GONG Yu,HUI Yan-ting,LIU Ruo-yu*
Author information +
History +

摘要

为筛选STAT1基因启动子区SNP及研究其对启动子功能元件的影响。选择品种差异较大的贵州荷斯坦奶牛和务川黑牛构建不同DNA池,直接测序筛选SNP位点。结果表明:STAT1基因5'调控区及第1外显子存在3个SNPs位点,分别为:T-537G、T-508A、C+10T。生物信息学软件预测得到STAT1基因核心启动子区和转录因子结合位点,SNP位点导致1个转录因子结合位点消失,而产生10个新的转录因子结合位点。CpG岛范围未受到突变位点影响,但STAT1基因RNA二级结构在突变后显著改变。研究结果为进一步确定STAT1启动子功能奠定试验基础。

Abstract

In order to screen the polymorphisms of STAT1 promoter in cattle and analyze the effect of SNPs on function elements of promoter,two cattle breeds (Wuchuan Black Cattle and Guizhou Holstein cow) with significant difference in breeds property were selected to construct DNA pools, SNP sites were screened by direct sequencing subsequently.The results showed that 3 single nucleotide polymorphisms (SNPs) were found in the 5' flanking region which included T-537G, T-508A and C+10T. Furthermore, bioinformatics tools were used to predict the core region of the promoter and identify various transcription factors binding sites. It demonstrated that 10 new transcription factors binding sites emerged while 1 previous transcription factors binding sites disappeared based on the SNPs found in this study, the remarkable change of secondary structure of RNA were also detected but the range of CpG islands were not influenced by the analysis of various softwares. This work could lay the experimental foundation for the identification of function of STAT1 promoter.

关键词

STAT1 / SNP / 启动子 / 贵州荷斯坦奶牛 / 务川黑牛

Key words

STAT1 / SNP / promoter / Guizhou Holstein Cow / Wuchuan Black Cattle

引用本文

导出引用
焦仁刚,杨永强,龚俞,惠嫣婷,刘若余*. STAT1基因启动子区多态性研究. 家畜生态学报. 2013, 34(1): 15-20
JIAO Ren-gang,YANG Yong-qiang,GONG Yu,HUI Yan-ting,LIU Ruo-yu*. Study on the Polymorphism of Promoter Region of STAT1 Gene in Cattle. Journal Of Domestic Animal Ecology. 2013, 34(1): 15-20

参考文献

[1]Rawlings J S, Rosler K M, Harrison D A. The JAK/STAT signaling pathway[J]. Journal of Cell Science, 2004, 117(8):1 2811 283.

[2]Deng L, Jiang J, Frank S J. Growth hormoneinduced JAK2 signaling and GH receptor downregulation: role of GH receptor intracellular domain tyrosine residues[J]. Endocrinology, 2012,153(5):2 3112 322.

[3]Calo V, Migliavacca M, Bazan V, et al. STAT proteins: from normal control of cellular events to tumorigenesis[J]. J Cell Physiol, 2003, 197(2):157168.

[4]Lanning N J, CarterSu C. Recent advances in growth hormone signaling[J]. Reviews in endocrine & metabolic disorders, 2006, 7(4):225235.

[5]Herrington J, Smit L S, Schwartz J, et al. The role of STAT proteins in growth hormone signaling[J]. Oncogene, 2000,19(21):2 5852 597.

[6]Cobanoglu O, Zaitoun I, Chang Y, et al. Effects of the signal transducer and activator of transcription 1 (STAT1) gene on milk production traits in Holstein dairy cattle[J]. Journal of Dairy Science,2006,89(11):4 4334 437.

[7]Tamura G, Yin J, Wang S, et al. ECadherin gene promoter hypermethylation in primary human gastric carcinomas[J]. Journal of the National Cancer Institute, 2000, 92(7):569573.

[8]Darnell Jr J E. STATs and gene regulation[J]. Science,1997,277(5332):1 6301 635.

[9]Kiu H, Nicholson S. Biology and significance of the JAK/STAT signalling pathways[J]. Growth factors,2012,30(2):88106.

[10]Khatib H, Huang W, Mikheil D, et al. Effects of signal transducer and activator of transcription (STAT) genes STAT1 and STAT3 genotypic combinations on fertilization and embryonic survival rates in Holstein cattle[J]. Journal of Dairy Science,2009,92(12):6 1866 191.

[11] 褚 敏, 昝林森. STAT1 基因单核苷酸多态性与中国荷斯坦奶牛产奶性状的相关性研究[J]. 畜牧兽医学报,2009,40(3):309314.

基金

贵州省重大科技专项计划项目(黔科合重大专项字[2011]6009号)

PDF(916 KB)

138

Accesses

0

Citation

Detail

段落导航
相关文章

/