大白鼠乳腺上皮细胞的体外培养与鉴定

索江华,江青东

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家畜生态学报 ›› 2012, Vol. 33 ›› Issue (5) : 50-52.
科学研究

大白鼠乳腺上皮细胞的体外培养与鉴定

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Culture and Identification of Rat Mammary Epithelial Cells

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摘要

试验选取泌乳期大白鼠乳腺组织,采用机械剪切结合胰酶消化的方法,在体外进行乳腺上皮细胞原代分离培养及鉴定。结果成功培养出大鼠原代乳腺上皮细胞,经过角蛋白免疫组化鉴定染色后,呈现阳性反应。显微镜下观察,细胞呈鹅卵石样;经多次传代,细胞增殖仍然长势很好。

Abstract

Take rat mammary tissue during lactation and use mechanical shear combined with trypsin digestion to isolate and culture mammary epithelial cells in vitro.The mammary epithe lial cells were successfully cultured,and after immunohisto chemial identification of keratin staining,it showed a positive reaction.Observed under the microscope,it was like cobblestone;after  many times of passage,cell proliferation was still growing well. It will provide a theoretical basis for future research work.

关键词

大鼠 / 乳腺上皮细胞 / 分离培养

Key words

 rat / mammary epithelial cells / isolate and culture

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导出引用
索江华,江青东. 大白鼠乳腺上皮细胞的体外培养与鉴定. 家畜生态学报. 2012, 33(5): 50-52
SUO Jiang-hua,JIANG Qing-dong. Culture and Identification of Rat Mammary Epithelial Cells. Journal Of Domestic Animal Ecology. 2012, 33(5): 50-52

参考文献

[1]郑玉才.水牛乳腺上皮细胞的体外培养[J].西南民族学院学报:自然科学版,1994,20(1):42-44.

[2]李   震,王   英,刘惠莉.牛乳腺上皮细胞的分离培养[J].上海农业学报,2000,16(3):25-28.

[3]Darcy K M,Shoemarker S E.Prolactin and epidermal growth factor regulation of theproliferation, morphogenesis,and functional differentiation of normal rat mammary epithelial cells in three dimensional culture[J]. J Cell Physiol,2005,157:136-164.

[4]Topper Y J,Freeman C S.Multiplehormone interactions in thedevel2opmental biologyof themammary gland[J].Physiol Rev, 2006,43:675-698.

[5]Tania Geman,Itamar Barash.Characterization of an epithelial cell line frombovine mammary gland[J].In Vitro Cell Dev Biol,2007;67:134-156.

[6]Zhang H Z,Bennett J M, Smith K T,et al.Estrogen mediates mammary epithelial cell proliferation in serum free culture indirectly viama mmary stroma derived hepatocyte growth factor[J].Endo,2006,113(9):212-225.

[7]鄂   征. 组织培养和分子细胞学技术[M]. 北京: 北京出版社, 2006:147.

[8]林桂娟,王   恬,陈才勇.机械破碎法分离奶牛乳腺上皮细胞的体外培养研究[J].畜牧与兽医,2004,36(7):148.

[9]薛庆善.体外培养的原理与技术[M].北京:科学出版社,2005: 326-367.

基金

河南省教育厅科普项目资助

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