FAN Yue-yuan,FU Guo-wen,ZAN Lin-sen,FU Chang-zhen, LI Wei-zhen,WANG Shao-qing
JOURNAL OF DOMESTIC ANIMAL ECOLOGY.
2012, 33(1):
14-18.
To reveal the SNP of PPARα gene exon7 and evaluate its relationship with carcass traits and meat traits in cattle, a total of 717 individuals from six breeds (QC, NY, JX, LX, XN and Angus) were genotyped by PCRSSCP and DNA sequencing in PPARα gene exon7 and analyzed its correlation of the SNP with some carcass traits and meat traits in 108 Qinchuan cattle. C→T mutation was detected at 184bp in PPARα gene exon7. Allele frequencies of PPARαE/F in the six breeds were 0.225/0.775,0.151/0.849, 0.125/0.875, 0.123/0.877, 0.070/0.930 and 0.157/0.783 for NY, QC, JX, Angus, LX and XN, respectively. Except for NY breed in moderate polymorphisms (PIC=0.288), other five breeds were in low genetic diversity in the locus(PIC<0.25). Correlation analysis showed that the mutation had significantly related to back fat thickness and carcass length in QC breed. Animals with the genotype FF had significantly higher traits than these with genotypes EE and EF in back fat thickness trait (P<0.05), and animals with the genotype FF had much higher than those with genotype EE (P<0.05) in carcass length trait. In conclusion, this locus is likely to be a major QTN or tightly linked that affect production traits, it can be a candidate molecular marker for the beef cattle breeding.